Experimental evaluation of kinetics and biochemical characteristics of MnO2 nanoparticles as high throughput peroxidase-mimetic nanomaterials
Vol 1, Issue 1, 2023
VIEWS - 155 (Abstract)
Abstract
Despite the well-known concepts on the intrinsic peroxidase-like activity of MnO2 nanoparticles, up to date, their biochemical and kinetics characteristics were not investigated, especially, the current information about their performances toward n-electron oxidation of 3, 3′-diaminobezedine for producing indamine polymers is on limitation. Therefore, herein, the MnO2 nanoparticles were synthesized by a simple low-cost co-precipitation method and then characterized by XRD, SEM, and DLS analysis. Besides, their peroxidase-like activity was evaluated upon standard peroxidase enzyme assay, revealing high intrinsic peroxidase-like activity for the as-mentioned MnO2 nanozymes. Considering their high intrinsic peroxidase-like activity, their optimal biochemical characteristics were quantified by probing the progress of n-electron irreversible oxidation of 3, 3′-diaminobezedine in the presence of MnO2 nanozymes as peroxidase mimics. The maximal activity of the as-mentioned MnO2 nanoparticles with high intrinsic peroxidase-like activity was observed when the pH and temperature of the reaction media were fixed over 3.0–6.0 and 23 ℃–25 ℃, in order, revealing very high pH and thermal stability of the as-prepared nanoparticles. The salt stability of these nanoparticles was also checked using NaCl as model salt, revealing that the nanozymatic activity was stable over a salt concentration as high as 3–7 M. In addition, the affinity constant (Km) and maximum velocity of the nanozyme-catalyzed oxidation of 3, 3′-diaminobezedine were found to be 1.6 mM and 47 nM sec−1, in turn.
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1. Hormozi Jangi SR, Akhond M. High throughput green reduction of tris (p-nitrophenyl) amine at ambient temperature over homogenous AgNPs as H-transfer catalyst. Journal of Chemical Sciences 2020; 132: 110. doi: 10.1007/s12039-020-01819-9
2. Hormozi Jangi SR. Low-temperature destructive hydrodechlorination of long-chain chlorinated paraffins to diesel and gasoline range hydrocarbons over a novel low-cost reusable ZSM-5@Al-MCM nanocatalyst: A new approach toward reuse instead of common mineralization. Chemical Papers 2023; 77: 4963–4977. doi: 10.1007/s11696-023-02834-z
3. Hormozi Jangi SR, Akhond M. Ultrasensitive label-free enantioselective quantification of D-/L-leucine enantiomers with a novel detection mechanism using an ultra-small high-quantum yield N-doped CDs prepared by a novel highly fast solvent-free method. Sensors and Actuators B: Chemical 2021; 339: 129901. doi: 10.1016/j.snb.2021.129901
4. He L, Chang C, Xue Q, et al. Bismuth nanoparticles decorated vertically arranged graphene as flexible electrodes for highly efficient detection of Pb(II) ions in water. Microchemical Journal 2023; 187: 108433. doi: 10.1016/j.microc.2023.108433
5. Zheng Z, Liang W, Lin R, et al. Facile synthesis of zinc indium oxide nanofibers distributed with low content of silver for superior antibacterial activity. Small Structures 2023; 4(4): 2200291. doi: 10.1002/sstr.202200291
6. Hormozi Jangi SR. Synthesis and characterization of magnesium-based metal-organic frameworks and investigating the effect of coordination solvent on their biocompatibility. Chemical Research and Nanomaterials 2023; 1(4): 1–9.
7. Zhang S, Ruan H, Xin Q, et al. Modulation of the biocatalytic activity and selectivity of CeO2 nanozymes via atomic doping engineering. Nanoscale 2023; 15(9): 4408–4419. doi: 10.1039/D2NR05742E
8. Hormozi Jangi SR, Dehghani Z. Spectrophotometric quantification of hydrogen peroxide utilizing silver nanozyme. Chemical Research and Nanomaterials 2023; 2(1): 15–23.
9. Yang D, Wang L, Jia T, et al. Au/Fe3O4-based nanozymes with peroxidase-like activity integrated in immunochromatographic strips for highly-sensitive biomarker detection. Analytical Methods 2023; 15(5): 663–674. doi: 10.1039/D2AY01815B
10. Akhond M, Hormozi Jangi SR, Barzegar S, Absalan G. Introducing a nanozyme-based sensor for selective and sensitive detection of mercury(II) using its inhibiting effect on production of an indamine polymer through a stable n-electron irreversible system. Chemical Papers 2020; 74: 1321–1330. doi: 10.1007/s11696-019-00981-w
11. Hormozi Jangi SR. Introducing a high throughput nanozymatic method for eco-friendly nanozyme-mediated degradation of methylene blue in real water media. Sustainable Chemical Engineering 2023; 4(2): 90–99. doi: 10.37256/sce.4220233204
12. Cao L. Immobilised enzymes: Science or art? Current Opinion in Chemical Biology 2005; 9(2): 217–226. doi: 10.1016/j.cbpa.2005.02.014
13. Homaei AA, Sariri R, Vianello F, Stevanato R. Enzyme immobilization: An update. Journal of Chemical Biology 2013; 6(4): 185–205. doi: 10.1007/s12154-013-0102-9
14. Hormozi Jangi SR, Akhond M. High throughput urease immobilization onto a new metal-organic framework called nanosized electroactive quasi-coral-340 (NEQC-340) for water treatment and safe blood cleaning. Process Biochemistry 2021; 105: 79–90. doi: 10.1016/j.procbio.2021.03.027
15. Klibanov AM. Enzyme stabilization by immobilization. Analytical Biochemistry 1979; 93: 1–25. doi: 10.1016/S0003-2697(79)80110-4
16. Hormozi Jangi SR. Determining kinetics parameters of bovine serum albumin-protected gold nanozymes toward different substrates. Qeios 2023. doi: 10.32388/07MWBC
17. Hormozi Jangi SR. Effect of daylight and air oxygen on nanozymatic activity of unmodified silver nanoparticles: Shelf-stability. Qeios 2023. doi: 10.32388/9RWVYI.3
18. Hormozi Jangi AR, Hormozi Jangi MR, Hormozi Jangi SR. Detection mechanism and classification of design principles of peroxidase mimic based colorimetric sensors: A brief overview. Chinese Journal of Chemical Engineering 2020; 28(6): 1492–1503. doi: 10.1016/j.cjche.2020.01.020
19. Bittencourt GA, de Souza Vandenberghe LP, Martínez-Burgos WJ, et al. Emerging contaminants bioremediation by enzyme and nanozyme-based processes—A review. iScience 2023; 26(6): 106785. doi: 10.1016/j.isci.2023.106785
20. Ahmadi-Leilakouhi B, Hormozi Jangi SR, Khorshidi A. Introducing a novel photo-induced nanozymatic method for high throughput reusable biodegradation of organic dyes. Chemical Papers 2023; 77(2): 1033–1046. doi: 10.1007/s11696-022-02542-0
21. Liu C, Fan W, Cheng WX, et al. Red emissive carbon dot superoxide dismutase nanozyme for bioimaging and ameliorating acute lung injury. Advanced Functional Materials 2023; 33(19): 2213856. doi: 10.1002/adfm.202213856
22. Zeng X, Ruan Y, Chen Q, et al. Biocatalytic cascade in tumor microenvironment with a Fe2O3/Au hybrid nanozyme for synergistic treatment of triple negative breast cancer. Chemical Engineering Journal 2023; 452, Part 2: 138422. doi: 10.1016/j.cej.2022.138422
23. Han J, Yoon J. Supramolecular nanozyme-based cancer catalytic therapy. ACS Applied Bio Materials 2020; 3(11): 7344–7351. doi: 10.1021/acsabm.0c01127
24. Hormozi Jangi SR, Akhond M. Synthesis and characterization of a novel metal-organic framework called nanosized electroactive quasi-coral-340 (NEQC-340) and its application for constructing a reusable nanozyme-based sensor for selective and sensitive glutathione quantification. Microchemical Journal 2020; 158: 105328. doi: 10.1016/j.microc.2020.105328
25. Yu R, Wang R, Wang Z, et al. Applications of DNA-nanozyme-based sensors. Analyst 2021; 146(4): 1127–1141. doi: 10.1039/D0AN02368J
26. Hormozi Jangi SR, Davoudli HK, Delshad Y, et al. A novel and reusable multinanozyme system for sensitive and selective quantification of hydrogen peroxide and highly efficient degradation of organic dye. Surfaces and Interfaces 2020; 21: 100771. doi: 10.1016/j.surfin.2020.100771
27. Xia HY, Li BY, Zhao Y, et al. Nanoarchitectured manganese dioxide (MnO2)-based assemblies for biomedicine. Coordination Chemistry Reviews 2022; 464: 214540. doi: 10.1016/j.ccr.2022.214540
28. Tang Y, Zheng S, Cao S, et al. Advances in the application of manganese dioxide and its composites as electrocatalysts for the oxygen evolution reaction. Journal of Materials Chemistry A 2020; 8(36): 18492–18514. doi: 10.1039/D0TA05985D
29. Wu J, Yang Q, Li Q, et al. Two-dimensional MnO2 nanozyme-mediated homogeneous electrochemical detection of organophosphate pesticides without the interference of H2O2 and color. Analytical Chemistry 2021; 93(8): 4084–4091. doi: 10.1021/acs.analchem.0c05257
30. Zou N, Wei X, Zong Z, et al. Preparation of manganese dioxide nanozyme as catalyst for electrochemical sensing of hydrogen peroxide. International Journal of Electrochemical Science 2021; 16(3): 210324. doi: 10.20964/2021.03.47
31. Hormozi Jangi SR, Akhond M, Absalan G. A novel selective and sensitive multinanozyme colorimetric method for glutathione detection by using an indamine polymer. Analytica Chimica Acta 2020; 1127: 1–8. doi: 10.1016/j.aca.2020.06.012
32. Yin Z, Ji Q, Wu D, et al. H2O2-responsive gold nanoclusters @ mesoporous silica @ manganese dioxide nanozyme for “off/on” modulation and enhancement of magnetic resonance imaging and photodynamic therapy. ACS Applied Materials & Interfaces 2021; 13(13): 14928–14937. doi: 10.1021/acsami.1c00430
33. Hormozi Jangi SR, Akhond M, Absalan G. A field-applicable colorimetric assay for notorious explosive triacetone triperoxide through nanozyme-catalyzed irreversible oxidation of 3, 3′-diaminobenzidine. Microchimica Acta 2020; 187(8): 431. doi: 10.1007/s00604-020-04409-1
34. Hormozi Jangi SR, Akhond M, Dehghani Z. High throughput covalent immobilization process for improvement of shelf-life, operational cycles, relative activity in organic media and enzymatic kinetics of urease and its application for urea removal from water samples. Process Biochemistry 2020; 90: 102–112. doi: 10.1016/j.procbio.2019.11.001
35. Temoçin Z, İnal M, Gökgöz M, Yiğitoğlu M. Immobilization of horseradish peroxidase on electrospun poly (vinyl alcohol)-polyacrylamide blend nanofiber membrane and its use in the conversion of phenol. Polymer Bulletin 2018; 75: 1843–1865. doi: 10.1007/s00289-017-2129-5
36. Ruginescu R, Enache M, Popescu O, et al. Characterization of some salt-tolerant bacterial hydrolases with potential utility in cultural heritage bio-cleaning. Microorganisms 2022; 10(3): 644. doi: 10.3390/microorganisms10030644
37. Hormozi Jangi SR, Akhond M. Introducing a covalent thiol-based protected immobilized acetylcholinesterase with enhanced enzymatic performances for biosynthesis of esters. Process Biochemistry 2022; 120: 138–155. doi: 10.1016/j.procbio.2022.06.004
38. Hormozi Jangi SR, Dehghani Z. Kinetics and biochemical characterization of silver nanozymes and investigating impact of storage conditions on their activity and shelf-life. Chemical Research and Nanomaterials 2023; 1(4): 25–33.
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