pisco_log Publisher

Biochemical characterization of enzyme-like silver nanoparticles toward nanozyme-catalysed oxidation reactions

Saeed Reza Hormozi Jangi

Article ID: 2170
Vol 1, Issue 1, 2023, Article identifier:

VIEWS - 220 (Abstract) 0 (PDF)


In this contribution, the biochemical characterization of enzyme-like nanosilvers was performed toward nanozyme-catalyzed oxidation reactions. In this regard, silver nanoparticles were synthesized via a simple chemical reduction method and then characterized by the TEM imaging method. Afterward, their enzyme-like activity was investigated toward catalysis of the oxidation reaction of 3,3’,5,5’-tetramethyl-benzidine (TMB) as one of the most popular peroxidase substrates. The results exhibited a specific nanozymatic activity as high as 5400 nM min−1 for the as-synthesized nanosilvers toward TMB oxidation. Due to the high enzyme-like activity of the as-prepared nanosilvers, their biochemical properties including pH, thermal, light, and shelf stability were characterized to explore more precisely describing their nanozymatic behavior. The results of thermal and pH stability studies showed that the as-prepared nanosilvers reveal their maximal enzyme-like activity at a wide temperature range of 25 ℃–35 ℃ and a pH range of 3.5–4.5, in order. Regarding the light stability and shelf-life studies, the results exhibited that 75% and 96% of the enzyme-like activity of the as-prepared nanozymes was saved after 7 days exposing visible light and 10 days of storage at 4 ℃ under dark conditions, in order.


biochemical characterization; enzyme-like nanosilvers; nanozyme-catalysed reactions; peroxidase mimics

Full Text:



1. Hormozi Jangi SR, Akhond M. High throughput green reduction of tris (p-nitrophenyl) amine at ambient temperature over homogenous AgNPs as H-transfer catalyst. Journal of Chemical Sciences 2020; 132: 1–8. doi: 10.1007/s12039-020-01819-9

2. Hormozi Jangi SR, Dehghani Z. Spectrophotometric quantification of hydrogen peroxide utilizing silver nanozyme. Chemical Research and Nanomaterials 2023; 2(1): 15–23.

3. Hormozi Jangi SR, Akhond M. Ultrasensitive label-free enantioselective quantification of d-/l-leucine enantiomers with a novel detection mechanism using an ultra-small high-quantum yield N-doped CDs prepared by a novel highly fast solvent-free method. Sensors and Actuators B: Chemical 2021; 339. doi: 10.1016/j.snb.2021.129901

4. Salehzadeh A, Sadat Shandiz A, Naeemi AS. Cytotoxicity effectiveness of biosynthesized silver nanoparticles on breast cancer T47D cell line, using macro algae Laurencia caspica extract. Journal of Ilam University of Medical Sciences 2018; 26(1): 52–61.

5. Hormozi Jangi SR. Synthesis and characterization of magnesium-based metal-organic frameworks and investigating the effect of coordination solvent on their biocompatibility. Chemical Research and Nanomaterials 2023; 1(4): 1–9.

6. Salata OV. Applications of nanoparticles in biology and medicine. Journal of Nanobiotechnology 2004; 2(1): 1–6. doi: 10.1186/1477-3155-2-3

7. Sánchez-Moreno P, De Vicente J, Nardecchia, S, et al. Thermo-sensitive nanomaterials: Recent advance in synthesis and biomedical applications. Nanomaterials 2018; 8(11): 935. doi: 10.3390/nano8110935

8. Hormozi Jangi SR, Akhond M, Absalan G. A field-applicable colorimetric assay for notorious explosive triacetone triperoxide through nanozyme-catalyzed irreversible oxidation of 3, 3′-diaminobenzidine. Microchimica Acta 2020; 187: 431. doi: 10.1007/s00604-020-04409-1

9. Aadil KR, Pandey N, Mussatto SI, Jha H. Green synthesis of silver nanoparticles using acacia lignin, their cytotoxicity, catalytic, metal ion sensing capability and antibacterial activity. Journal of Environmental Chemical Engineering 2019; 7(5). doi: 10.1016/j.jece.2019.103296

10. Jangi SRH, Akhond M. Synthesis and characterization of a novel metal-organic framework called nanosized electroactive quasi-coral-340 (NEQC-340) and its application for constructing a reusable nanozyme-based sensor for selective and sensitive glutathione quantification. Microchemical Journal 2020; 158. doi: 10.1016/j.microc.2020.105328

11. Jangi SRH, Akhond M, Absalan G. A novel selective and sensitive multinanozyme colorimetric method for glutathione detection by using an indamine polymer. Analytica Chimica Acta 2020; 1127: 1–8. doi: 10.1016/j.aca.2020.06.012

12. Jangi ARH, Jangi MRH, Jangi SRH. Detection mechanism and classification of design principles of peroxidase mimic based colorimetric sensors: A brief overview. Chinese Journal of Chemical Engineering 2020; 28(6): 1492–1503. doi: 10.1016/j.cjche.2020.01.020

13. Hormozi Jangi SR. Low-temperature destructive hydrodechlorination of long-chain chlorinated paraffins to diesel and gasoline range hydrocarbons over a novel low-cost reusable ZSM-5@ Al-MCM nanocatalyst: A new approach toward reuse instead of common mineralization. Chemical Papers 2023; 1–15. doi: 10.1007/s11696-023-02834-z

14. Akhond M, Hormozi Jangi SR, Barzegar S, Absalan G. Introducing a nanozyme-based sensor for selective and sensitive detection of mercury (II) using its inhibiting effect on production of an indamine polymer through a stable n-electron irreversible system. Chemical Papers 2020; 74: 1321–1330. doi: 10.1007/s11696-019-00981-w

15. Jangi SRH, Davoudli HK, Delshad Y, et al. A novel and reusable multinanozyme system for sensitive and selective quantification of hydrogen peroxide and highly efficient degradation of organic dye. Surfaces and Interfaces 2020; 21. doi: 10.1016/j.surfin.2020.100771

16. Ahmadi-Leilakouhi B, Hormozi Jangi SR, Khorshidi A. Introducing a novel photo-induced nanozymatic method for high throughput reusable biodegradation of organic dyes. Chemical Papers 2023; 77(2): 1033–1046. doi: 10.1007/s11696-022-02542-0

17. Zhou Y, Liu B, Yang R, Liu J. Filling in the gaps between nanozymes and enzymes: Challenges and opportunities. Bioconjugate Chemistry 2017; 28(12): 2903–2909. doi: 10.1021/acs.bioconjchem.7b00673

18. Jangi SRH, Akhond M. High throughput urease immobilization onto a new metal-organic framework called nanosized electroactive quasi-coral-340 (NEQC-340) for water treatment and safe blood cleaning. Process Biochemistry 2021; 105: 79–90. doi: 10.1016/j.procbio.2021.03.027

19. Jangi SRH, Akhond M. Introducing a covalent thiol-based protected immobilized acetylcholinesterase with enhanced enzymatic performances for biosynthesis of esters. Process Biochemistry 2022; 120: 138–155. doi: 10.1016/j.procbio.2022.06.004

20. Jangi SRH, Akhond M, Dehghani Z. High throughput covalent immobilization process for improvement of shelf-life, operational cycles, relative activity in organic media and enzymatic kinetics of urease and its application for urea removal from water samples. Process Biochemistry 2020; 90: 102–112. doi: 10.1016/j.procbio.2019.11.001

21. Kavousi M, Fatemi D. The effect of Spirulina Microalgae extract on Bcl-2 Anti-Apoptotic Gene Expression in Brain Cancer Cell Line. Pars Journal of Medical Sciences 2022; 17(3): 17–23. doi: 10.52547/JMJ.17.3.17

22. Shanmugaraj K, Ilanchelian M. Colorimetric determination of sulfide using chitosan-capped silver nanoparticles. Microchimica Acta 2016; 183: 1721–1728. doi: 10.1007/s00604-016-1802-y

23. Moulaie S, Mirzaie A, Aliasgari E. Antibacterial and anticancer activities of silver nanoparticles fabricated by the Artemisia scoparia extract against lung cancer cell line (A549). KAUMS Journal (FEYZ) 2018; 22(5): 487–496.

24. Jangi SRH. Introducing a high throughput nanozymatic method for eco-friendly nanozyme-mediated degradation of methylene blue in real water media. Sustainable Chemical Engineering 2023; 4: 90–99. doi: 10.37256/sce.4220233204

25. Jangi SRH. Determining kinetics parameters of bovine serum albumin-protected gold nanozymes toward different substrates. Qeios 2023. doi: 10.32388/07MWBC

26. Hormozi Jangi SR. A comparative study on kinetics performances of BSA-gold nanozymes for nanozyme-mediated oxidation of 3,3’,5,5’-Tetramethylbenzidine and 3,3’-Diaminobenzidine. Biochemistry and Molecular Biology Journal 2023; 9: 21. doi: 10.36648/2471-8084-9.03.21

(220 Abstract Views, 0 PDF Downloads)


  • There are currently no refbacks.

Copyright (c) 2023 Saeed Reza Hormozi Jangi

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.