This study examined the impacts of land use on the physical and chemical properties of soils of land use types along agroforestry and agricultural landscapes in a rainforest zone of Nigeria. The land use systems are forest, agroforestry, fallow, and ornamental plant fields in addition to permanent crop fields (cocoa, oil palm, and citrus) and annual crop fields (maize). Profile pits were dug on the land use types and samples were collected 0–20 cm and 20–50 cm for laboratory analysis. Soil samples were collected from undisturbed soil and profile pits for bulk density and moisture content determination following standard analytical procedures. Among the land use types, physical properties (sand, clay, soil bulk density) and chemical properties (soil pH, SOC, total N, P, K, Ca, Mg, and CEC) differed significantly. Bulk density, pH, SOC, total, and stocks of SOC and N differed statistically for 0–20 and 20–50 cm soil depths with downward increases in N and SOC stocks along sampling depth. Permanent croplands (forest and agroforestry fields) had higher soil pH, SOC, total N, and CEC, while arable crop fields had relatively lower pH, SOC, TN, P, K, Ca, Mg, and CEC. Arable fields had significantly lower C and N stocks within 50 cm compared with permanent crop fields, which may be attributed to continuous tillage by the smallholder farmers and soil erosion-enhanced SOC and N removal from top soil. For both permanent and annual crop fields, SOC and total N stocks ranged from 5.75 to 3.12 kg/m² for 0–20 cm depths and 2.44 to 1.93 kg/m² for deeper (20–50 cm) layers. Relative to forest soil, stocks of SOC in the surface soils (0–20 cm) decreased in the order: agroforestry > ornamental plant field > cocoa > fallow land > citrus > oil palm > annual cropping system. Following this decreasing order, soil deterioration indices are equivalent to 27% > 28% > 30% > 31% > 32% > 34% > 38% compared with forest soil, respectively. Strong significant correlations (p < 0.05) were observed between SOC and TN stocks and some soil properties (bulk density, clay contents, pH, and CEC) with R² values ranging from 1.0 to 0.85. It is concluded that the soil's physical and chemical properties and carbon storage potential differed among the land uses of the study site.