Effects of biochar immobilized microorganisms on the enzyme activity and remediation of petroleum hydrocarbon in contaminated soil
Vol 4, Issue 1, 2023
VIEWS - 63 (Abstract)
Abstract
The high-temperature pyrolysis oxidation method was adopted to prepare reed biochar, and reed biochar was used as the immobilized material to load the dominant petroleum hydrocarbon degrading bacteria, and the effects of biochar immobilized microorganisms on the remediation effect and enzyme activity of petroleum hydrocarbon contaminated soil were studied. The results showed that after 40 days of remediation of petroleum hydrocarbon contaminated soil by immobilized microorganisms with reed biochar, the removal rate of petroleum hydrocarbon in soil reached 55.01%, which was significantly higher than that of single biochar (45.82%) and blank treatment group (24.83%). It was also found that biochar immobilized microorganisms could significantly improve the activities of dehydrogenase, catalase, urease and polyphenol oxidase in soil. The use of biochar immobilized microorganism technology can not only improve the remediation efficiency of petroleum hydrocarbon contaminated soil, but can also significantly increase the soil biological enzyme activity.
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1. Wanglinchang Impact of petroleum hydrocarbon development on environment and Countermeasures [D]. Qingdao: Ocean University of China, 2009
2. Liuwuxing, luoyongming, tengying, et al Ecological risk assessment and bioremediation of petroleum contaminated soil II Study on physical and chemical properties and microbial ecological changes of oil contaminated soil [J]. Journal of soil, 2007,4 (5): 848-853
3. Qiao Xingguo, lifayun, zhang Ying, et al Effects of freezing and thawing on enzyme activity and water-soluble carbon in oil contaminated soil [J]. Journal of Agricultural Environmental Sciences, 2008,4 (3): 914-919
4. Zhan Yan Harm of soil petroleum hydrocarbon pollution in China and its control countermeasures [J]. Environmental pollution and prevention, 2008,30 (3): 91-93
5. ALEBELEVE O,OPEOLU B,JACKSON V. Polycyclic aromatic hydrocarbons:A critical review of environmental occurrence and bioremediation[J]. Environmental Management,2017,60(4): 1-26.
6. ZHANG B, zhang L, zhang X. Bioremediation of petroleum hydrocarbon contaminated soil by petroleumdegrading bacteria immobilized on biochar[J]. RSC Advances, 2019, 9: 35304.
7. Cao Zhongli Study on bioremediation effect and toxicity change of petroleum contaminated soil [D]. Xi'an: Xi'an University of architecture and technology, 2017
8. Xin Lei Experimental study on step-by-step Fenton chemical oxidation of heavily petroleum contaminated soil [D]. Xi'an: Xi'an University of architecture and technology two thousand and twelve
9. Tang Jing Diversity analysis of heavy metal resistant actinomycetes in soil of sewage irrigation area in the northern suburbs of Xi'an [d]. Xianyang: Northwest University of agriculture and forestry science and technology, 2009
10. Luguilan, wangshijie, guoguanlin, et al Effect of peat intensification on bioremediation effect of aged oil sludge [J]. Journal of environmental engineering technology, 2011,1 (5): 389-395
11. Yang Qian, wumanli, nie Maixi, et al Bioremediation technology and microbial ecological effect of petroleum hydrocarbon contaminated soil [J]. Environmental Science, 2015, 36 (5): 1856-1863
12. XIE W, li R, li X, et al. Different responses to soil petroleum contamination in monocultured and mixed plant systems[J]. Ecotoxicology & Environmental Safety,2018,161: 763-768.
13. Zhangxiuxia, bai Xuejing, xunana, et al Study on Influencing Factors of oil contaminated soil remediation by immobilized microorganisms [J]. Journal of environmental engineering, 2013, 7 (3): 1156-1162
14. Zhangxiuxia, gengchunxiang, fang Miaomiao, et al Application of immobilized microorganisms in bioremediation of oil contaminated soil [J]. Journal of Petroleum (petroleum processing), 2008,4: 409-414.
15. XU Y, lu M. Bioremediation of crude oil-contaminated soil: Comparison of different biostimulation and bioaugmentation treatments[J]. Journal of Hazardous Materials,2010,183(1-3): 395-401.
16. Wangxuhui, chaoqunfang, xuxin, et al Laboratory simulation study on bioremediation of petroleum contaminated soil [J]. Journal of environmental engineering, 2012, 6 (5): 1663-1668
17. Wangyanjie, lifayun, rongxiangmin, et al Remediation of petroleum hydrocarbon contaminated soil by combined application of biomass materials and nutrients [J]. Journal of Agricultural Environmental Sciences, 2018,37 (2): 232-238
18. Zhuwenying, tangjingchun Remediation effect of wheat straw biochar on petroleum hydrocarbon contaminated soil [J]. Journal of agricultural resources and environment, 2014,31 (3): 259-264
19. Wangyanjie Study on the mechanism and effect of Combined Bioremediation of petroleum hydrocarbon contaminated soil by biochar immobilized microorganisms and plants [D]. Changsha: Hunan Agricultural University, 2017
20. Ruan Zhiyong Screening and identification of oil degrading strains and preliminary study on their oil degradation characteristics [D]. Beijing: Chinese Academy of Agricultural Sciences, 2006
21. Guan Yinsong Soil enzymes and their research methods [M]. Beijing: Agricultural Press, 1986
22. Zhoulikai Soil enzymology [M]. Beijing: Science Press, 1989
23. Wang Jing, zhangzhongzhi, su Youming, et al Study on rhizosphere effect of phytoremediation of petroleum hydrocarbon contaminated soil [J]. Journal of petrochemical colleges and universities, 2008, 21 (6): 36-40
24. Wuweilin, zhangxiuxia, shan Baolai, et al Effects of different disposal methods on physical, chemical and biological properties of petroleum hydrocarbon contaminated soil [J]. Journal of Petroleum (petroleum processing), 2010,26 (5): 831-834
25. Gao Feng, yang Fengjun, wu Xia, et al Effects of biochar application on organic matter content and enzyme activity in rhizosphere soil of Chinese cabbage [J]. Soil bulletin, 2019, 50 (1): 103-108
26. GRAHAM D W, smith V H, LAW K P. Effects of nitrogen and phosphoroussupply on hexadecane biodegradation in soil systems[J]. Water,Air,and Soil Pollution,1999,111(1/4): 1-18.
27. Zhangxiuxia, zhangshoujuan, zhang Han, et al Effects of immobilized microorganisms on biological characteristics of oil contaminated soil [J]. Journal of Petroleum (petroleum processing), 2015,31 (1): 112-118
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