Open Journal Systems

Immunoanalytical approaches for the control of xenobiotics and biotoxins in foodstuffs

Josep V. Mercader, Antonio Abad-Somovilla Abad-Somovilla, Consuelo Agulló, Antonio Abad-Fuentes

Article ID: 1969
Vol 2, Issue 2, 2021

DOI: https://doi.org/10.54517/aas.v2i2.1969
VIEWS - 61 (Abstract)

Abstract

Effective control of food quality and safety requires analytical methods that guarantee the reliable determination of any substance potentially harmful to the consumer that may be present in the food prior to its distribution and marketing. One of the analytical approaches that contributes to guarantee this objective encompasses a series of techniques that have in common the use of antibodies as essential elements for the detection of the target analyte, and which together are called immunochemical methods. This article aims to provide a basic overview of the biochemical principles underlying these technologies and their advantages and limitations in the determination of chemical contaminants, residues and additives in food matrices. The last part discusses some of our initiatives in this field that have resulted in commercially available rapid kits after transferring the corresponding technology to the industrial sector.

Keywords

Immunoassay; Rapid methods; ELISA; Immunoreactive strip; Hapten; Antibody; Food quality and safety; Contaminant; Residue.

Full Text:

PDF

References

1. Abad-Fuentes, A., Esteve-Turrillas, F. A., Agulló, C., Abad-Somovilla, A. and Mercader, J. V. (2012). Development of competitive enzyme-linked immunosorbent assays for boscalid determination in fruit juices. Food Chemistry, 135 (1),pp. 276-284. https://doi.org/10.1016/j. foodchem.2012.04.090.

2. Esteve-Turrillas, F. A., Abad-Somovilla, A., Quiñones-Reyes, G., Agulló, C., Mercader, J. V. and Abad-Fuentes, A. (2015). Monoclonal antibody-based immunoassays for cyprodinil residue analysis in QuEChERS-based fruit extracts. Food Chemistry, 187, pp. 530-536. https://doi. org/10.1016/j.foodchem.2015.04.119.

3. Esteve-Turrillas, F. A., Mercader, J. V., Agulló, C., Abad-Somovilla, A. and Abad-Fuentes, A. (2015). Moiety and linker site heterologies for highly sensitive immunoanalysis of cyprodinil in fermented alcoholic beverages. Food Control, 50, pp. 393-400. https://doi.org/10.1016/j.foodcont.2014.09.023.

4. Esteve-Turrillas, F. A., Parra, J., Abad-Fuentes, A., Agulló, C., Abad-Somovilla, A. and Mercader, J. V. (2010). Hapten synthesis, monoclonal antibody generation, and development of competitive immunoassays for the analysis of picoxystrobin in beer. Analytica Chimica Acta, 682 (1-2), pp. 93-103. https://doi. org/10.1016/j.aca.2010.09.042.

5. González-Techera, A., Varell, L., Last, J. A., Hammock, B. D. and González-Sapienza, G. (2007). Phage anti-immune complex assay: General strategy for non-competitive immunodetection of small molecules. Analytical Chemistry, 79 (20), pp. 7799-7806. https://doi.org/10.1021/ ac071323h

6. Landsteiner, K. and Simms, S. (1923). Production of heterogenetic antibodies with mixtures of the binding part of the antigen and protein. Journal of Experimental Medicine, 38 (2), pp. 127-138. https://doi.org/10.1084/jem.38.2.127

7. López-Moreno, R., Mercader, J. V., Agulló, C., Abad-Somovilla, A. and Abad-Fuentes, A. (2014). Immunoassays for trifloxystrobin analysis. Part I. Rational design of regioisomeric haptens and production of monoclonal antibodies. Food Chemistry, 152, pp. 230-236. https://doi. org/10.1016/j.foodchem.2013.11.150.

8. López-Puertollano, D., Mercader, J. V., Agulló, C., Abad-Somovilla, A. and Abad-Fuentes, A. (2018). Novel hapten and monoclonal antibodies with subnanomolar affinity for a classical analytical target, ochratoxin A. Scientific Reports, 8 (1), 9761. https://doi.org/10.1038/s41598- 018-28138-x

9. Mercader, J. V., Agulló, C., Abad-Somovilla, A. and Abad-Fuentes, A. (2011). Synthesis of site-heterologous haptens for highaffinity anti-pyraclostrobin antibody generation. Organic & Biomolecular Chemistry, 9 (5), pp. 1443-1453. https:// doi.org/10.1039/c0ob00686f.

10. Mercader, J. V., López-Moreno, R., EsteveTurrillas, F. A., Abad-Somovilla, A. and Abad-Fuentes, A. (2014). Immunoassays for trifloxystrobin analysis. Part II. Assay development and application to residue determination in food. Food Chemistry, 162, pp. 41-46. https://doi. org/10.1016/j.foodchem.2014.04.053.

11. Montalbetti, C. A. G. N. and Falque, V. (2005). Amide bond formation and peptide coupling. Tetrahedron, 61 (46), pp. 10827-10852. https://doi.org/10.1016/j. tet.2005.08.031.

12. Parra, J., Mercader, J. V., Agulló, C., AbadSomovilla, A. and Abad-Fuentes, A. (2012). Generation of anti-azoxystrobin monoclonal antibodies from regioisomeric haptens functionalized at selected sites and development of indirect competitive immunoassays. Analytica Chimica Acta, 715, pp. 105-112. https://doi. org/10.1016/j.aca.2011.12.014.

13. Ramón-Azcón, J., Sánchez-Baeza, F., Sanvicens, N. and Marco, M. P. (2009). Development of an enzyme-linked immunosorbent assay for determination of the miticide bromopropylate. Journal of Agricultural and Food Chemistry, 57 (2), pp. 375-384. https://doi.org/10.1021/ jf802821n

14. Sanvicens, N., Pichon, V., Hennion, M. C. and Marco, M. P. (2003). Preparation of antibodies and development of an enzymelinked immunosorbent assay for determination of dealkylated hydroxytriazines. Journal of Agricultural and Food Chemistry, 51 (1), pp. 156-164. https:// doi.org/10.1021/jf025640v.

15. Suárez-Pantaleón, C., Mercader, J. V., Agulló, C., Abad-Somovilla, A. and Abad-Fuentes, A. (2008). Production and characterization of monoclonal and polyclonal antibodies to forchlorfenuron. Journal of Agricultural and Food Chemistry, 56 (23), pp. 11122-11131. https://doi. org/10.1021/jf802261x.

16. Suárez-Pantaleón, C., Mercader, J. V., Agulló, C., Abad-Somovilla, A. and AbadFuentes, A. (2011). Forchlorfenuron-mimicking haptens: from immunogen design to characterization by hierarchical clustering analysis. Organic & Biomolecular Chemistry, 9 (13), pp. 4863-4872. https://doi.org/10.1039/c1ob05190c.

17. Vallejo, R. P., Bogus, E. R. and Mumma, R. O. (1982). Effects of hapten structure and bridging groups on antisera specificity in parathion immunoassay development. Journal of Agricultural and Food Chemistry, 30 (3), pp. 572-580. https:// doi.org/10.1021/jf00111a040.