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Impact of six digestion methods on the measurement of polystyrene microplastics in organisms using fluorescence intensity
Vol 3, Issue 1, 2022
Issue release: 31 December 2022
VIEWS - 6673 (Abstract)
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Abstract
Microplastic pollution has emerged as a significant global environmental issue, raising considerable concern. To assess the biological impacts of microplastics, precise quantification within organisms is essential. Fluorescence intensity is a common method for measuring microplastics, but biological sample digestion—a crucial pre-treatment step—can potentially degrade the microplastics, affecting fluorescence readings and causing discrepancies between measured and actual values. This study investigates six widely used digestive agents: KOH, naoh, H2O2, HNO3, HNO3: hcl, and HNO3: HClO4. The effects of these digestion methods on microplastic fluorescence intensity and surface morphology were evaluated to determine the most effective protocol. The results indicate that KOH digestion (100 g·L-1, 60℃) has the least impact on fluorescence intensity and preserves the microplastics' surface morphology, whereas the other five methods caused varying degrees of fluorescence reduction and surface damage (such as aggregation, bubbles, scratches, and depressions). Furthermore, the KOH digestion method achieved a recovery rate of ≥96.3%±0.5% when used to extract microplastics from biological samples, demonstrating its suitability for analyzing fluorescent microplastics in such samples.
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Prof. Sivanesan Subramanian
Anna University, India
Prof. Pascal Lorenz
University of Haute Alsace, France
Dortmund University of Technology, Germany.
Interests: Mass spectrometry, Molecular Structural Analysis, Methodology; Application; Biological, Environmental and Food samples.
