
Asia Pacific Academy of Science Pte. Ltd. (APACSCI) specializes in international journal publishing. APACSCI adopts the open access publishing model and provides an important communication bridge for academic groups whose interest fields include engineering, technology, medicine, computer, mathematics, agriculture and forestry, and environment.

MiR-342-5p Alleviates Inflammatory Responses in Sepsis-Induced AKI by Targeting PFN1
Vol 38, Issue 5, 2024
Download PDF
Abstract
Background: The development of several human diseases has been linked to microRNA-342-5p (miR-342-5p). However, its specific function in the inflammatory reactions associated with sepsis-triggered acute kidney injury (AKI) remains unexplored. This study aims to delve into the role of miR-342-5p in the progression of sepsis-induced AKI. Methods: To understand the underlying mechanism of miR-342-5p in sepsis, we established sepsis models through induction with lipopolysaccharide (LPS). The cell proliferation and apoptosis were assessed using the 5-ethynyl-2′-deoxyuridine (EdU) method and flow cytometry, respectively. Bioinformatics analysis was employed to predict the downstream binding target of miR-342-5p, and their interaction was confirmed through a dual-luciferase reporter gene assay. The rescue tests were conducted to further explore the action pathway of miR-342-5p. Results: The administration of LPS significantly decreased the HK2 cell proliferation (p < 0.05), and increased cell apoptosis. Additionally, there was a notable downregulation in miR-342-5p expression accompanied by an elevated level of inflammatory factor (p < 0.05). Furthermore, the overexpression of miR-342-5p effectively attenuated the LPS-induced inflammatory response in HK2 cells (p < 0.05). Profilin-1 (PFN1) emerged as a pivotal player in sepsis pathogenesis, serving as a critical downstream target of miR-342-5p. Interestingly, rescue experiments demonstrated that the stimulatory effects of miR-342-5p mimics were counteracted by the overexpression of PFN1 in sepsis. Conclusions: MiR-342-5p alleviates inflammatory responses in sepsis by targeting PFN1.
Keywords
References
Supporting Agencies
Copyright (c) 2024 Yongshun Li, Zhen Chen
This site is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).

Medical Genetics, University of Torino Medical School, Italy

Department of Biomedical, Surgical and Dental Sciences, University of Milan, Italy