Chemical Analysis, Antioxidant, Toxicology, and Antinociceptive Effects of Moroccan Cannabis sativa Essential Oil

Hamid Kabdy, Abdelmounaim Baslam, Anass Belbachir, Stefania Garzoli, Abderrahman Chait

Article ID: 7896
Vol 38, Issue 3, 2024
DOI: https://doi.org/10.23812/j.biol.regul.homeost.agents.20243803.165
Received: 20 March 2024; Accepted: 20 March 2024; Available online: 20 March 2024; Issue release: 20 March 2024

Abstract

Background: Cannabis sativa L. is an annual plant from the Cannabaceae family. In Morocco, it has a long-standing traditional use. This study aimed to perform a chemical analysis of the Cannabis sativa essential oil (CSEO) and assess its acute toxicity, antioxidant properties, and analgesic effects. Method: Chemical analysis was conducted on the essential oil extracted from Cannabis sativa using gas chromatography-mass spectrometry (GC/MS) to identify its components. Subsequently, we carried out in vitro assessments of antioxidant activities using the 2,2-diphenyl-1-picrylhydrazyl radical scavenging method (DPPH), the iron reduction method, and the β-carotene assay. Concurrently, we conducted investigations into the antinociceptive activity using three distinct animal models: the writhing test, the formalin test, and the hot plate test, as well as the acute toxicity by determining the lethal dose 50 (LD50). Results: The GC/MS analysis of the CSEO allowed the identification of its major compounds, namely (E)-caryophyllene α-humulene and caryophyllene oxide. The antioxidant activity was also significant due to its ability to scavenge DPPH (IC50 = 1.16 ± 0.08 mg/mL) and reduce iron potency (IC50 = 1.49 ± 0.46 mg/mL) and IC50 = 1.8 ± 0.2 mg/mL for β-carotene/linoleic acid assay. Acute toxicity (LD50) was evaluated at 42.46 mg/kg, indicating a relatively high tolerance, while a significant analgesic effect was observed, persisting for 120 minutes. Conclusions: The study provides promising results for the essential oil of the Moroccan variety of Cannabis sativa (C. sativa), showing its potential therapeutic properties as an antioxidant, and analgesic agent.


Keywords

antioxidant;analgesic;Cannabis sativa;chemical analysis;essential oil


References

Supporting Agencies



Copyright (c) 2024 Hamid Kabdy, Abdelmounaim Baslam, Anass Belbachir, Stefania Garzoli, Abderrahman Chait




This site is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).