ISYNA1 is a New Diagnostic and Prognostic Marker of Colorectal Cancer that Promotes Proliferation and Migration of Malignant Cells

Abstract

Background: Colorectal cancer (CRC), a malignant tumor, shows the highest incidence and mortality rates in China and worldwide. Inositol-3-phosphate synthase 1 (ISYNA1) is the core enzyme involved in inositol biosynthesis. However, the effect of ISYNA1 on human cancer development, particularly CRC, has been poorly studied. Our study aimed to explore the precise molecular targets related to the diagnosis and treatment of CRC. Methods: We evaluated ISYNA1 expression and gene control networks in CRC applying sequencing data. We measured ISYNA1 mRNA and protein degrees in CRC cell lines applying quantitative real-time polymerase chain response and immunofluorescence imaging. While knocking down ISYNA1 expression in HCT116 and SW480 cell lines by small interfering RNA (siRNA) transfection, we verified this by cell viability, colony formation, and migration assays. Results: Evaluation on the cancer genome atlas (TCGA) database demonstrated the overexpression of ISYNA1 in CRC, and survival discussion demonstrated that patients with great ISYNA1 expression had a lower prognosis (p < 0.05). According to univariate and multivariate Cox regression models, ISYNA1 is an independent prognostic element for CRC. ISYNA1 affects tumor progression via multiple cancer-related signaling pathways. Real-time quantitative polymerase chain reaction (RT-qPCR) results demonstrated that HCT116 and SW480 cells displayed greatly higher ISYNA1 expression than DLD1 and HT29 cells (p < 0.05). ISYNA1 expression in HCT116 and SW480 cell lines was knocked down. Compared to the control group, ISYNA1-down-regulated CRC cell lines demonstrated significantly reduced proliferation, colony formation, and migration (p < 0.05). Conclusions: In conclusion, ISYNA1 may be a novel prognostic biomarker for CRC, laying the basis for further studies the effect of ISYNA1 on CRC occurrence and development.