
Asia Pacific Academy of Science Pte. Ltd. (APACSCI) specializes in international journal publishing. APACSCI adopts the open access publishing model and provides an important communication bridge for academic groups whose interest fields include engineering, technology, medicine, computer, mathematics, agriculture and forestry, and environment.

Piperine Exhibits Anticancer Potential by Inhibiting the Cell Cycle Progression in Vitro
Vol 38, Issue 1, 2024
Abstract
Background: Leukaemia is a blood-related cancer that is the leading cause of death worldwide. Piperine, a spicy alkaloid found in the spice Piper nigrum, has multifunctional properties. However, its anticancer effects against human chronic myeloid leukemia (CML) cells are yet to be investigated. The present study aims to investigate the anticancer potential of piperine using the human CML cell line KCL22. Methods: The anticancer effects of piperine were investigated through 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT) assay, generation of reactive oxygen species (ROS), cellular apoptosis, and cell cycle arrest. Additionally, in silico molecular docking was utilized to predict cell death by detecting the binding interactions of piperine with surface proteins of leukemia cells, specifically death receptors 4 (DR4) and death receptors 5 (DR5). Results: Data in the MTT assay showed that piperine treatment inhibited the cellular proliferation of KCL22 cells in a dose-dependent manner. Furthermore, nuclear condensation and ROS generation significantly increased when KCL22 cells were treated with piperine. Further study on cell cycle progression showed that piperine treatment inhibited cell cycle progression at G2/M and S phases. In silico molecular docking study showed that piperine has a good binding interaction with both DR4 and DR5. No violation of Lipinskis rule of five was observed. Piperine exhibited drug-likeness properties without toxicity. Conclusions: These findings suggest that piperine may have potential as an anticancer drug against human chronic myeloid leukemia.
Keywords
References
Supporting Agencies
Copyright (c) 2024 Abdulaziz S. Alothaim
This site is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).

Medical Genetics, University of Torino Medical School, Italy

Department of Biomedical, Surgical and Dental Sciences, University of Milan, Italy