Endothelial Cell-Derived Interleukin-6 Facilitated Colorectal Cancer Cell Migration and Invasion by Regulating Osteopontin in a Paracrine Manner

Qianyuan Li; Zhiyun Pan; Xiukou Zhou

doi:10.23812/j.biol.regul.homeost.agents.20243801.59

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Asia Pacific Academy of Science Pte. Ltd. (APACSCI) specializes in international journal publishing. APACSCI adopts the open access publishing model and provides an important communication bridge for academic groups whose interest fields include engineering, technology, medicine, computer, mathematics, agriculture and forestry, and environment.

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2025

Vol 39, No 3 (2025)

Vol 39, No 2 (2025)

Vol 39, No 1 (2025)

Featured Articles

Macrophages in the pathogenesis of psoriasis and anti-psoriatic nanotherapies

Psoriasis is a common, chronic, and inflammatory skin disease. Macrophages account for about 61.3% of the inflammatory cells infiltrating psoriatic lesions. Modulating macrophage polarization, inhibiting their infiltration, and targeting the secretion of inflammatory factors and associated inflammatory pathways by these cells can alleviate psoriasis symptoms and inflammation. Moreover, nanomaterials as novel drug carriers, offer unique advantages such as large surface area, easy modification, high biocompatibility, good biodegradability, enhanced systemic adsorption, etc. Nanomaterials have great potential for efficient drug delivery and release, as well as improving therapeutic efficacy while reducing adverse effects. By systematically addressing the role of macrophages in psoriasis pathogenesis and the potential of nanomaterials in treating psoriasis through modulating macrophages, this review enhances our understanding of the disease mechanism and holds promise for novel therapeutic breakthroughs and advancements in the future treatment of psoriasis.

Endothelial Cell-Derived Interleukin-6 Facilitated Colorectal Cancer Cell Migration and Invasion by Regulating Osteopontin in a Paracrine Manner

Qianyuan Li, Zhiyun Pan, Xiukou Zhou

Article ID: 7788
Vol 38, Issue 1, 2024

DOI: https://doi.org/10.23812/j.biol.regul.homeost.agents.20243801.59

Received: 20 January 2024; Accepted: 20 January 2024; Available online: 20 January 2024; Issue release: 20 January 2024

Abstract

Background: Patients with metastatic colorectal cancer (CRC) generally suffer from poor prognosis. Therefore, there is an urgent need to further delineate the mechanism of metastasis in CRC, which is of clinical significance for the development of more targeted therapy. This study aimed to explore the potential role of endothelial cells-derived interleukin-6 (IL-6) in CRC metastasis. Methods: Human umbilical vein endothelial cell (HUVEC)-conditioned medium and CRC-conditioned medium were prepared, with the latter being the control. Enzyme-linked immunosorbent assay was employed to detect IL-6 levels in HUVEC-conditioned or CRC-conditioned culture media. CRC cells were separately cultured in both media to investigate the effect of HUVECs on CRC metastasis using cell counting kit-8 assay, scratch test and Transwell assay. The role of HUVEC-derived IL-6 in CRC cells was investigated using rescue assay. The expressions of the osteopontin protein and the proteins in glycogen synthase kinase-3β (GSK3β)-β-catenin signaling pathways were determined by Western blotting. To confirm the mediating role of osteopontin in the relationship between IL-6 and CRC, the expression of osteopontin in CRC cells was up-regulated. Results: IL-6 level was higher in HUVEC-conditioned medium than in CRC-conditioned medium (p < 0.001). CRC cells in the HUVEC-conditioned medium showed enhanced viability, migration and invasion (p < 0.05). HUVEC-secreted IL-6 facilitated malignant phenotypes and activated osteopontin and GSK3β-β-catenin signaling pathway of CRC cells in a dose-dependent manner (p < 0.05). Moreover, osteopontin overexpression contributed to CRC metastasis in vitro and activated GSK3β-β-catenin signaling pathway (p < 0.001), while such effect was abrogated by IL-6 silencing (p < 0.001). Conclusion: HUVEC-derived IL-6 facilitated the metastasis of CRC cells by regulating osteopontin in a paracrine manner.

Keywords

colorectal cancer;metastasis;paracrine;interleukin-6;osteopontin;glycogen synthase kinase-3β-β-catenin signaling pathway

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Dr. Fabio Malavasi

Medical Genetics, University of Torino Medical School, Italy

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Prof. Marcello Iriti

Department of Biomedical, Surgical and Dental Sciences, University of Milan, Italy

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2025-02-01

Notice of Change in Publication Schedule

In addition to the first issue that has already been published by the original publisher, there will be four more issues released this year, with scheduled publication dates in March, June, September, and December respectively.

2025-01-21

Notice: Ownership Transfer of the Journal

Starting from Volume 39 Issue 2 (2025), the ownership of Journal of Biological Regulators and Homeostatic Agents (ISSN: 0393-974X (P); 1724-6083 (O)) will be transferred from Biolife Sas to Asia Pacific Academy of Science Pte. Ltd. As of 21 January 2025, authors should make submissions to the new journal system and follow the author guidelines. Asia Pacific Academy of Science Pte. Ltd. will take over the publication of manuscripts being processed.

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