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Ilexgenin A Alleviated the Endoplasmic Reticulum Stress and Oxidative Stress-Induced Apoptosis in Amyloid-Beta25-35-Treated PC12 Cells
Vol 37, Issue 6, 2023
Abstract
Background: Ilexgenin A is a natural triterpenoid compound with a certain role in inflammation, atherosclerosis and tumor, and can regulate endoplasmic reticulum stress. The relevant effects of Ilexgenin A on Alzheimers disease (AD) are discussed in the present study. Methods: Following the indicated transfection and treatment of Ilexgenin A (0.1, 1, 10 μmol/L) or 25 μmol/L amyloid-beta (Aβ)25-35, the assays of both 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) and flow cytometry were adopted to estimate the viability and apoptosis of PC12 cells. Meanwhile, the levels of superoxide dismutase (SOD), malondialdehyde (MDA), and Reactive Oxygen Species (ROS) were determined by the assay kits of SOD and MDA and flow cytometry. Besides, the expressions of estrogen receptor 1 (ESR1), B-cell lymphoma-2 (Bcl-2), BCL2-Associated X protein (Bax), Glucose-Regulated Protein 78 (GRP78), C/EBP homologous protein (CHOP), and nuclear/cytoplasmic nuclear factor kappa B (NF-κB) p65 were quantified by Western blot or quantitative real-time polymerase chain reaction (qRT-PCR) as needed. Results: Aβ25-35 decreased viability, promoted apoptosis, suppressed Bcl-2 and ESR1 protein expression levels, and enhanced the Bax protein level of PC12 cells (p < 0.001). Conversely, Ilexgenin A reversed the Aβ25-35 effects on these aspects in PC12 cells (p < 0.05). Besides, Aβ25-35 diminished SOD level and downregulated cytoplasmic NF-κB p65 protein expression while increased the levels of MDA, ROS and upregulated protein expressions of GRP78, CHOP, Caspase-12, nuclear NF-κB p65 (p < 0.001). On the other hand, Ilexgenin A exerted the opposite effects, which were overturned following the downregulation of ESR1 (p < 0.001). Conclusions: In PC12 cells with the intervention of Aβ25-35, which mimicked an in vitro AD model, Ilexgenin could alleviate the initiated oxidative stress and endoplasmic reticulum stress-induced apoptosis via upregulating ESR1.
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Copyright (c) 2023 Jijuan Zhang, Ningning Bu, Hong Zhang, Haiyun Shao, Chenghe Sun
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Medical Genetics, University of Torino Medical School, Italy

Department of Biomedical, Surgical and Dental Sciences, University of Milan, Italy