Knockdown of LCTL, A Diagnostic Biomarker in Patients with HNSCC, Promotes Apoptosis of HNSCC Cells via the PI3K/Akt Pathway

Yumei Huang; Dianguo Zhao; Changcheng Ding; Xiangrui Ma; Honghai Fu

doi:10.23812/j.biol.regul.homeost.agents.20233705.275

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Asia Pacific Academy of Science Pte. Ltd. (APACSCI) specializes in international journal publishing. APACSCI adopts the open access publishing model and provides an important communication bridge for academic groups whose interest fields include engineering, technology, medicine, computer, mathematics, agriculture and forestry, and environment.

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Macrophages in the pathogenesis of psoriasis and anti-psoriatic nanotherapies

Psoriasis is a common, chronic, and inflammatory skin disease. Macrophages account for about 61.3% of the inflammatory cells infiltrating psoriatic lesions. Modulating macrophage polarization, inhibiting their infiltration, and targeting the secretion of inflammatory factors and associated inflammatory pathways by these cells can alleviate psoriasis symptoms and inflammation. Moreover, nanomaterials as novel drug carriers, offer unique advantages such as large surface area, easy modification, high biocompatibility, good biodegradability, enhanced systemic adsorption, etc. Nanomaterials have great potential for efficient drug delivery and release, as well as improving therapeutic efficacy while reducing adverse effects. By systematically addressing the role of macrophages in psoriasis pathogenesis and the potential of nanomaterials in treating psoriasis through modulating macrophages, this review enhances our understanding of the disease mechanism and holds promise for novel therapeutic breakthroughs and advancements in the future treatment of psoriasis.

Knockdown of LCTL, A Diagnostic Biomarker in Patients with HNSCC, Promotes Apoptosis of HNSCC Cells via the PI3K/Akt Pathway

Yumei Huang, Dianguo Zhao, Changcheng Ding, Xiangrui Ma, Honghai Fu

Article ID: 7331
Vol 37, Issue 5, 2023

DOI: https://doi.org/10.23812/j.biol.regul.homeost.agents.20233705.275

Received: 8 June 2023; Accepted: 8 June 2023; Available online: 8 June 2023; Issue release: 8 June 2023

Abstract

Background: The Klotho family comprises a series of newly identified anti-aging genes, including α-Klotho (KL), β-Klotho (KLB), and γ-Klotho (LCTL). However, the role of LCTL in the progression of head and neck squamous cell carcinoma (HNSCC) has not been elucidated. Methods: The expression of KL/KLB/LCTL and gene variations in patients with HNSCC were analyzed using the CbioPortal and The Cancer Genome Atlas Program (TCGA) databases. Moreover, KL/KLB/LCTL expression levels in HNSCC tissues were determined using real-time quantitative PCR (Polymerase Chain Reaction) (qPCR) and compared with those in paired non-cancerous adjacent tissues. Cell viability and apoptosis of HNSCC cells were assessed using methylthiazoletetrazolium (MTT) and fluorescence-activated cell sorting assays, respectively. The levels of LCTL and key proteins in the phosphatidylinositol-3-kinase (PI3K)/serine/threonine-protein kinase (Akt) signaling pathway were determined using western blotting. Results: The KL/KLB level was decreased, and the LCTL level was elevated in patients with HNSCC compared with that in normal controls. In addition, the LCTL level was increased in HNSCC cell lines compared with that in human keratinocyte (HACAT) cells. Interference with LCTL significantly suppressed the proliferation of HNSCC cells, promoted apoptosis of SCC (Squamous Cell Carcinoma) 15 cells, and increased the expression of (BCL2 Associated X) Bax and p21 by inhibiting PI3K and Akt phosphorylation. Conclusions: The data suggest that LCTL is a potential diagnostic biomarker for patients with HNSCC. Interference with LCTL significantly suppressed the viability of HNSCC cells and promoted apoptosis of SCC15 cells via the PI3K/Akt signaling pathway.

Keywords

LCTL;HNSCC;diagnosis;apoptosis

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Medical Genetics, University of Torino Medical School, Italy

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Department of Biomedical, Surgical and Dental Sciences, University of Milan, Italy

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2025-02-01

Notice of Change in Publication Schedule

In addition to the first issue that has already been published by the original publisher, there will be four more issues released this year, with scheduled publication dates in March, June, September, and December respectively.

2025-01-21

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Starting from Volume 39 Issue 2 (2025), the ownership of Journal of Biological Regulators and Homeostatic Agents (ISSN: 0393-974X (P); 1724-6083 (O)) will be transferred from Biolife Sas to Asia Pacific Academy of Science Pte. Ltd. As of 21 January 2025, authors should make submissions to the new journal system and follow the author guidelines. Asia Pacific Academy of Science Pte. Ltd. will take over the publication of manuscripts being processed.

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