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Effects of Thyroid Peroxidase Antibody (TPO-Ab) on Apoptosis and Proliferation of Ovarian Granulosa Cells
Vol 37, Issue 5, 2023
Abstract
Objective: This work aims to observe the toxic effects of thyroid peroxidase antibody (TPO-Ab) on human ovarian granulosa cells (KGNs) and to investigate the mechanism of endoplasmic reticulum (ER) stress in TPO-Ab-induced apoptosis in KGNs. Methods: TPO-Ab (2 μg/mL, 4 μg/mL, and 8 μg/mL) was used to treat the KGNs. Cell counting kit-8 (CCK-8) and flow cytometry were performed to evaluate the effects of TPO-Ab on ovarian granulosa cell proliferation and apoptosis. The expression of specific proteins was detected by western blot. The levels of estradiol (E2) and progesterone were measured by enzyme-linked immunosorbent assay (ELISA). Results: TPO-Ab inhibited KGN proliferation and promoted apoptosis in a concentration-dependent manner. TPO-Ab reduced the levels of E2 and progesterone in KGNs. TPO-Ab increased the expressions of ER stress (ERS)-related proteins and decreased the expression level of Bcl-2. Conclusions: TPO-Ab repressed KGN proliferation and apoptosis by promoting ERS and eventually inducing apoptosis.
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Copyright (c) 2023 Haiyan Yu, Changzhou Li, Xiaoming Niu, Mingying Fan, Shiwei Huo
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Medical Genetics, University of Torino Medical School, Italy
Department of Biomedical, Surgical and Dental Sciences, University of Milan, Italy