Effect of Shugan Jianpi Qingre Recipe on Plasma Metabolomics in Functional Dyspepsia: An Animal Study

Abstract

Objective: To explore the mechanism of Shugan Jianpi Qingre Fang in the treatment of functional dyspepsia (FD) by using ultra-high-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (UHPLC-QTOFMS). Methods: 30 clean healthy Wistar rats were randomly divided into 3 groups, of 10 rats: Normal Group, Model Group and Shugan Jianpi Qingre Group. The functional dyspepsia model was made by “Guos clamping tail method” and “irregular feeding method”. The model time was 7 days. The rats in the traditional Chinese Medicine Group were given 13.98 G/kg/D of Shugan Jianpi Qingre Fang, and the rats in the normal group and the model group were given the same volume of distilled water once daily for 14 days. On the 22nd day of the experiment, the rats in each group were euthanized, blood was collected, plasma samples were prepared and metabonomics analysis was performed, to estimate the differential metabolites (15 potential biomarkers, such as Choline, proline, Taurine, glutamic acid, Glutathione, l-citrulline, Tryptophan, and eicosapentaenoic acid (EPA)) and find the main differential metabolic pathways. Results: In the model group, the level of estrone significantly increased whereas the level of the other metabolites significantly decreased. After the intervention of “Shugan Jianpi Qingre Fang”, the level of Choline, proline, Taurine, glutamic acid and mannose in the plasma of the model group significantly regressed. It is better to include figures and p values. Conclusions: Shugan Jianpi Qingre Fang may play an important role in the treatment of Functional Dyspepsia, by regulating glucose metabolism, energy metabolism, affecting nerve signal transduction and enhancing antioxidant capacity. Compound Chinese medicine has the characteristics of multi-angle and multi-target.