Amygdalin Induced Mitochondria-Mediated Apoptosis of Hypopharyngeal Carcinoma

Chao Long, Yongchun Li, Qiangyou Shi, Fan Guo, Zhipeng Mi, Xueliang Shen, Ruixia Ma

Article ID: 7278
Vol 37, Issue 4, 2023
DOI: https://doi.org/10.23812/j.biol.regul.homeost.agents.20233704.226
Received: 9 May 2023; Accepted: 9 May 2023; Available online: 9 May 2023; Issue release: 9 May 2023

Abstract

Purpose: Amygdalin is a natural extract effective in cancer inhibition, though its effects on hypopharyngeal squamous cell carcinoma (HSCC) have not been investigated. This study aims to investigate the in vitro anti-hypopharyngeal carcinoma activity of amygdalin and its potential mechanism. Methods: The hypopharyngeal squamous cell carcinoma cell lines (FADU and TU212) were treated with amygdalin. The proliferation of hypopharyngeal carcinoma was tested by mitochondria targeting sequence (MTS) assay. Furthermore, Annexin V-fluorescein isothiocyanate/propidine iodide (Annexin V-FITC/PI) double staining, immunofluorescence and mitochondrial membrane potential (MMP) assays were taken to test apoptosis. Apoptotic mRNA transcript levels and protein expression were evaluated using real-time quantitative PCR (qPCR) and western blot (WB). Results: FADU and TU212 cells were treated with amygdalin. MTS assay showed that amygdalin inhibited the proliferation of hypopharyngeal carcinoma cells. Annexin V-FITC/PI double staining, immunofluorescence and MMP detection assays were carried out and confirmed that amygdalin induced apoptosis in hypopharyngeal carcinoma cells. In addition, qPCR and WB analysis suggested that amygdalin exerted a carcinogenic anti-hypopharyngeal effect by mediating the mitochondrial apoptosis pathway. Conclusions: Amygdalin can inhibit the proliferation of hypopharyngeal carcinoma cells and promote apoptosis of hypopharyngeal carcinoma cells by inducing the mitochondrial apoptosis pathway. Amygdalin is a potential chemotherapeutic agent for hypopharyngeal carcinoma.


Keywords

amygdalin;hypopharyngeal squamous cell carcinoma;apoptosis;mitochondrial membrane potential;intrinsic apoptosis pathway


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