Application Value of PCR, LAMP and Bacterial Culture in the Diagnosis of Bacillary Dysentery and Correlation with Immune Indexes

Abstract

Background: Accurate and rapid detection methods can provide a scientific basis for improving the coincidence rate of clinical diagnosis and etiological diagnosis of bacillary dysentery. This study aims to explore the application value of polymerase chain reaction (PCR), loop-mediated isothermal amplification (LAMP) and bacterial culture in diagnosing bacillary dysentery. Methods: The feces samples and serum of 90 patients with diarrhea were collected as the observation group, and feces samples and serum of 90 healthy persons with physical examination in the same period were enrolled as the control group. PCR, LAMP, and bacterial culture were used to detect bacterial dysentery and to compare the advantages of the three methods. Results: The diagnostic accuracy of PCR and LAMP was much higher than that of bacterial culture. The sensitivity of PCR, LAMP and bacterial culture was 61.11%, 58.89% and 11.11%, and the specificity was 71.11%, 77.78% and 8.89%, respectively. The expression levels of interleukin (IL)-8, IL-12 p40 and procalcitonin (PCT) in the observation group were higher than in the control group. Levels of the cluster of differentiation (CD)3⁺, CD4⁺, and CD4⁺/CD8⁺ in the observation group were all lower than in control. Conclusions: After a comprehensive comparison of the three methods, PCR and LAMP showed better specificity and sensitivity, which could be better applied in detecting Shigella.