Potential Exosomal miRNAs and Associated Molecular Mechanism in Multiple Myeloma

Guoqing Zhu, Yanping Ma, Xue Fu, Xiaofei Ai, Gang An, Hui Cheng

Article ID: 7239
Vol 37, Issue 4, 2023
DOI: https://doi.org/10.23812/j.biol.regul.homeost.agents.20233704.185
Received: 9 May 2023; Accepted: 9 May 2023; Available online: 9 May 2023; Issue release: 9 May 2023

Abstract

Objectives: This study evaluates the potential exosomal microRNAs (miRNAs) and associated molecular mechanisms during the progression of multiple myeloma (MM). Methods: Serum exosomes RNA isolation and transcriptome sequencing were assessed in MM patients and healthy individuals. The differentially expressed miRNAs (DEmiRNAs) were assessed depending on the sequencing data. miRNA-mRNA relations analysis, enrichment analysis, protein-protein interaction (PPI) network investigation, miRNAs and mRNAs and competing endogenous RNAs (ceRNA) network analysis were performed. Results: 665 miRNAs were identified from the raw exosome RNA sequencing data. A total of 46 DEmiRNAs and 419 miRNA-mRNA interaction relations were found in the two groups. The mRNAs in miRNA-mRNA interactions were gathered in functions of salivary gland development and pathways like PI3K (phosphoinositide 3-kinases)-Akt (protein kinase B) signaling. Finally, a ceRNA network constructed with several lncRNA-miRNA-mRNA interactions including SPACA6P-AS-miR-125b-5p-signal transducer and activator of transcription 3 (STAT3) was created. Conclusions: Salivary gland development function and PI3K-Akt signaling pathway enriched by exosome-based gene contributed to the development of MM. Moreover, exosome-derived miR-125b-5p and STAT3 in SPACA6P-AS-miR-125b-5p-STAT3 axis may potentially be used as biomarkers for the progression of MM.


Keywords

exosomes;multiple myeloma;enrichment analysis;ceRNA


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