Correlation of miRNAs Gene Polymorphisms with Serum Lipoprotein Expression and Bone Metastasis of Primary Lung Cancer

Abstract

Objectives: To investigate microRNAs (miRNAs) gene polymorphisms and serum lipoprotein expression levels in patients with primary lung cancer metastasis, and to provide new ideas and basis for the diagnosis and treatment of primary lung cancer. Methods: From July 2016 to August 2018, 400 patients who were diagnosed with primary lung cancer were selected as the experimental group. Among them, 280 patients with bone metastasis were recorded as group A, including 200 male patients and 80 female patients, aged 28–85 years. The remaining 120 patients without bone metastasis were recorded as group B, including 90 males and 30 females, aged 29–88 years. For the clinical data characteristics of these patients, the plasma miRNAs were extracted and detected, and single nucleotide polymorphisms (SNPs) associated with miRNAs were selected and detected. The expression levels of serum lipoprotein in the plasma of the included patients were detected. Patients with bone metastasis underwent magnetic resonance imaging (MRI) examination based on artificial intelligence full convolutional neural network (CNN). Results: Statistically, there were no significant differences in the distribution of gender, smoking history, and drinking history among primary lung cancer patients with and without bone metastasis (p > 0.05). The expression levels of miR-145, miR-101, and miR-126 in primary lung cancer patients with bone metastasis were 1.22, 1.42, and 1.09, respectively. The expression levels of miR-145, miR-101, and miR-126 in primary lung cancer patients without bone metastasis were 0.83, 0.91, and 0.51, respectively. Therefore, the relative expression levels of 3 miRNAs in the plasma of patients in group A were increased (p < 0.05). Among the 8 ecological sites of the three miRNAs, there was a statistical difference in the genotype frequency distribution of rs353291 between the bone metastasis group and the non-metastatic group (χ2 = 4.676, p = 0.043). The expression levels of serum lipoprotein (a) in primary lung cancer patients with bone metastasis were raised compared with patients with non-bone metastasis. Conclusions: This study demonstrated that miRNAs gene polymorphisms and serum lipoprotein expression levels could provide new insights and a basis for the diagnosis and treatment of primary lung cancer.