Key Potential Long Non-Coding RNAs Related to Plaque Instability in Patients with Carotid Artery Stenosis Analyzed by RNA Sequencing

Ying Deng, Xueguang Lin, Bo Chen, Jindong Tong, Weijun Shi, Bo Wang, Bo Yu, Jingdong Tang

Article ID: 7142
Vol 37, Issue 2, 2023
DOI: https://doi.org/10.23812/j.biol.regul.homeost.agents.20233702.70
Received: 11 March 2023; Accepted: 11 March 2023; Available online: 11 March 2023; Issue release: 11 March 2023

Abstract

Aim: We aimed to characterize the profiles of long non-coding RNA (lncRNA) and mRNA associated with plaque instability in carotid artery stenosis (CAS). Methods: Three stable and three unstable plaque tissues were collected for RNA sequencing to filtrate differential expressed lncRNAs (DE-lncRNAs) and mRNAs (DE-mRNAs). Identified genes were then used for functional analyses, and six DE-lncRNAs as well as six inflammation-related DE-mRNAs were verified using RT-qPCR (reverse transcription quantitative polymerase chain reaction). Results: Using sequencing, we identified 920 DE-mRNAs (639 upregulated and 281 downregulated mRNAs) and 178 DE-lncRNAs (128 upregulated and 50 downregulated lncRNAs) in unstable plaques. According to functional analysis, these identified genes were obviously enriched in “signal transduction”, “phagocytosis”, “PI3K-Akt (phosphatidylinositol 3 kinase (PI3K)/protein kinaseB) signaling pathway”, “sphingolipid transporter activity”, “TNF (tumor necrosis factor) signaling pathway”, “ICAM-3 (intercellular adhesion molecule-3) receptor activity”, “Jak-STAT (Janus kinase-signal transducer and activator of transcription) signaling pathway”, “interleukin-6 receptor binding”, “Th17 (T helper cell 17) cell differentiation”, “HIF-1 (hypoxia-inducible factor-1) signaling pathway”, “Th1 (helper T lymphocyte 1) and Th2 (helper T lymphocyte 2) cell differentiation”, “NF-κB (nuclear factor kappa-B) signaling pathway”, and “sphingolipid signaling pathway”. Compared to stable plaque tissues, lncRNAs FAM30A, MIAT, and LUCAT1 were significantly upregulated in unstable plaque tissues (p < 0.05), whereas XIST (X inactive-specific transcript) and DLX6-AS1 were markedly downregulated (p < 0.05), based on RT-qPCR results. These findings corroborated expression patterns of lncRNA sequencing data. Compared with stable plaque tissues, expression of CCL19 (chemokine (C-C motif) ligand 19), IL6 (interleukin 6), CCL21 (chemokine (C-C motif) ligand 21), and IL18R1 (interleukin 18 receptor 1) was significantly upregulated, whereas SFRP5 (secreted frizzled related protein 5) was downregulated in unstable plaque tissues. Conclusions: DE-lncRNAs FAM30A, MIAT, LUCAT, XIST, and DLX6-AS1 may be potential targets for plaque instability, and the PI3K-Akt, Jak-STAT, NF-κB, TNF, and HIF-1 signaling pathways may have connection with plaque instability in patients with CAS.


Keywords

plaque stability;RNA sequencing;carotid artery stenosis;differential expressed lncRNAs


References

Supporting Agencies



Copyright (c) 2023 Ying Deng, Xueguang Lin, Bo Chen, Jindong Tong, Weijun Shi, Bo Wang, Bo Yu, Jingdong Tang




This site is licensed under a Creative Commons Attribution 4.0 International License (CC BY 4.0).