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Effects of Osteoglycin (OGN) on the Growth and Metastatic Ability of Epithelial Ovarian Cancer
Vol 37, Issue 1, 2023
Abstract
Objective: The existing literature suggests that osteoglycin (OGN) expression may be closely related to tumor transformation and invasion and metastasis, but there is a lack of evidence for OGN involvement in invasion and metastasis in ovarian cancer. The aim of this study was to investigate the relationship between OGN expression levels and the extent of ovarian cancer growth and metastasis through in vitro and in vivo studies. Methods: Microarrays of tumor tissue from 122 patients with epithelial ovarian cancer were used to detect OGN expression. The pMSCV-OGN lentiviral vector and its negative control were transfected into SKOV3 cells and the transfection efficiency was detected using Western blotting. The effects of OGN overexpression on the migration and invasion of SKOV3 cells were examined using wound healing tests and Transwell® assays. Cell Counting Kit 8 (CCK8) assay was used to detect SKOV3 cell proliferation. In addition, a mouse model of lung metastasis of ovarian cancer was established by tail vein injection of SKOV3 cells overexpressing OGN to investigate the effect of overexpressing OGN on the growth and metastasis of ovarian cancer by calculating the survival time, tumor volume, and the number of lung surface metastases of mice. Results: Ovarian cancer patients with low OGN expression are more likely to metastasize. OGN does not affect cell proliferation, but overexpression of OGN significantly inhibits the migration and invasion of SKOV3 cells. Overexpression of OGN in combination with cisplatin therapy significantly prolonged survival and inhibited tumor growth in mice with lung metastases from ovarian cancer. Conclusions: Overexpression of OGN prolonged the survival of tumor-bearing mice by inhibiting invasion and metastasis of ovarian cancer cells. OGN may become an important potential target for the prediction and control of ovarian cancer metastasis.
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Copyright (c) 2023 Jing-min Yu, Min-yi Yang, Xia Zhao, Jing-hui Hu
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Medical Genetics, University of Torino Medical School, Italy

Department of Biomedical, Surgical and Dental Sciences, University of Milan, Italy