Phosphatase and Tensin Homolog-Engineered Bone Marrow-Derived Mesenchymal Stem Cells Inhibited Alveolar Bone Resorption and Osteoclast Formation in Experimental Periodontitis Rats

Jia Hou; Ling Li; Xuetong Zhang; Yuan An; Liang Zhao

doi:10.23812/j.biol.regul.homeost.agents.20233701.4

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Asia Pacific Academy of Science Pte. Ltd. (APACSCI) specializes in international journal publishing. APACSCI adopts the open access publishing model and provides an important communication bridge for academic groups whose interest fields include engineering, technology, medicine, computer, mathematics, agriculture and forestry, and environment.

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Macrophages in the pathogenesis of psoriasis and anti-psoriatic nanotherapies

Psoriasis is a common, chronic, and inflammatory skin disease. Macrophages account for about 61.3% of the inflammatory cells infiltrating psoriatic lesions. Modulating macrophage polarization, inhibiting their infiltration, and targeting the secretion of inflammatory factors and associated inflammatory pathways by these cells can alleviate psoriasis symptoms and inflammation. Moreover, nanomaterials as novel drug carriers, offer unique advantages such as large surface area, easy modification, high biocompatibility, good biodegradability, enhanced systemic adsorption, etc. Nanomaterials have great potential for efficient drug delivery and release, as well as improving therapeutic efficacy while reducing adverse effects. By systematically addressing the role of macrophages in psoriasis pathogenesis and the potential of nanomaterials in treating psoriasis through modulating macrophages, this review enhances our understanding of the disease mechanism and holds promise for novel therapeutic breakthroughs and advancements in the future treatment of psoriasis.

Phosphatase and Tensin Homolog-Engineered Bone Marrow-Derived Mesenchymal Stem Cells Inhibited Alveolar Bone Resorption and Osteoclast Formation in Experimental Periodontitis Rats

Jia Hou, Ling Li, Xuetong Zhang, Yuan An, Liang Zhao

Article ID: 7082
Vol 37, Issue 1, 2023

DOI: https://doi.org/10.23812/j.biol.regul.homeost.agents.20233701.4

Received: 8 February 2023; Accepted: 8 February 2023; Available online: 8 February 2023; Issue release: 8 February 2023

Abstract

Background: This study aimed to assess the biological efficacy of phosphatase and tensin homolog-engineered bone marrow-derived mesenchymal stem cells (PTEN eBM-MSCs) in periodontitis treatment. Methods: Ligation was used to construct the experimental periodontitis model, and Sprague-Dawley rats were randomly divided into four groups (10 rats per group). Another 13 normal rats were used as controls. Micro-computer tomography (CT) was applied to measure the distance between the cemento-enamel junction (CEJ) and the palatal alveolar bone crest (ABC). Hematoxylin and eosin (HE) staining was used to observe the inflammatory infiltration and pathological morphology, and tartrate-resistant acid phosphatase (TRAP) staining evaluated the number of osteoclasts. Besides, enzyme-linked immunosorbent assay (ELISA) was used to measure the levels of tumor necrosis factor (TNF)-α, interleukin-17, and interleukin-10. Results: Cell viability and the expressions of anti-alkaline phosphatase (ALP), anti-osteocalcin, and anti-Runt-related transcription factor 2 (RUNX2) in the PTEN eBM-MSC group was significantly increased (p < 0.05). Periodontal ligament fibers in the periodontitis group were disordered, and osteoclast number, CEJ-ABC distance, TNF-α and interleukin-17 contents were increased compared to control rats, while interleukin-10 levels were declined (p < 0.05). Compared with the periodontitis group, the other three groups showed improvement of periodontal tissue damage compare with the periodontitis group, also osteoclast number, CEJ-ABC distance, TNF-α and interleukin-17 levels were decreased, while interleukin-10 levels were increased (p < 0.05). The greatest therapeutic effect was seen in the periodontitis+PTEN eBM-MSC group. Conclusions: PTEN eBM-MSCs inhibited inflammation, osteoclast formation and alveolar bone resorption in rats with experimental periodontitis.

Keywords

PTEN-engineered BM-MSCs;periodontitis;alveolar bone resorption;osteoclast formation;inflammatory

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Medical Genetics, University of Torino Medical School, Italy

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Department of Biomedical, Surgical and Dental Sciences, University of Milan, Italy

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2025-02-01

Notice of Change in Publication Schedule

In addition to the first issue that has already been published by the original publisher, there will be four more issues released this year, with scheduled publication dates in March, June, September, and December respectively.

2025-01-21

Notice: Ownership Transfer of the Journal

Starting from Volume 39 Issue 2 (2025), the ownership of Journal of Biological Regulators and Homeostatic Agents (ISSN: 0393-974X (P); 1724-6083 (O)) will be transferred from Biolife Sas to Asia Pacific Academy of Science Pte. Ltd. As of 21 January 2025, authors should make submissions to the new journal system and follow the author guidelines. Asia Pacific Academy of Science Pte. Ltd. will take over the publication of manuscripts being processed.

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