c-Myc Positively Enhances RPL34 Expression to Regulate Osteosarcoma Cell Proliferation

Abstract

Background: Ribosomal protein L34 (RPL34) is a member of the L34E ribosomal protein family containing zinc finger domains. This protein plays a key role in regulating the apoptosis, cell cycle progression and proliferation of various cancers including osteosarcoma (OS). Based on the analysis of the University of California Santa Cruz (UCSC) database, it was found that c-Myc may play an important role in regulating the expression of RPL34. Methods: In this study, we explored the mechanism by which c-Myc regulates RPL34 expression. The expression levels of c-Myc and RPL34 were measured by qRT-PCR and Western blot. Luciferase reporter assays and chromatin immunoprecipitation (ChIP) were used to analyse the binding site of c-Myc and RPL34. Results: The results showed that c-Myc may bind to the E-box region in the RPL34 promoter to regulate RPL34 expression. The results indicated that c-Myc regulates OS cell proliferation through RPL34. This research may provide new ideas for targeted therapy of OS. Conclusions: c-Myc regulates osteosarcoma cell proliferation through RPL34.