Biodegradable microcarriers as cell delivery vehicle for in vivo transplantation and magnetic resonance monitoring


Article ID: 6837
Vol 25, Issue 2S1, 2011
DOI: https://doi.org/10.54517/jbrha6837
Received: 9 July 2011; Accepted: 9 July 2011; Available online: 9 July 2011; Issue release: 9 July 2011

Abstract

Microcarrier culture systems offer an attractive method for cell amplification and as delivery vehicle. At the same time, super paramagnetic iron oxide (SPIO) nanoparticles represent a unique in vivo tracking system, already approved for clinical use. In our study, we tested the combination of clinically approved microcarriers and SPIO nanoparticles for cell-construct delivery and subsequent tracking after implantation. In order to mimic better a clinical setting, biodegradable macroporous microcarriers were employed as an alternative approach to expand human primary chondrocytes in a dynamic culture system for subsequent direct transplantation. In addition, cellseeded microcarriers were labeled with SPIO nanoparticles to evaluate the benefits of cell-constructs tracking with magnetic resonance. In vivo subcutaneous implants were monitored for up to 3 weeks and orthotopic implantation was simulated and monitored in ex vivo osteochondral defects.


Keywords

microcarriers;iron oxide particles;magnetic resonance;in vivo transplantation;cartilage


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