Extracorporeal shock waves induce osteogenic differentiation of human bone-marrow stromal cells

A Notarnicola, G Vicenti, G Maccagnano, F Silvestris, P Cafforio, B Moretti

Article ID: 6587
Vol 30, Issue 4S1, 2016
DOI: https://doi.org/10.54517/jbrha6587
Received: 8 January 2017; Accepted: 8 January 2017; Available online: 8 January 2017; Issue release: 8 January 2017

Abstract

The effects of treatment with shock waves (SW) on osteoblastic cells have already been described. Furthermore, the effects of treatment with SW are also determined by the contextual stimulation of other cell lines, in particular of mesenchymal cells. This is the first experimental study of stimulation of a human mesenchymal stem cell line, taken from bone marrow, using SW (electromagnetic device), with two energy levels. The results showed a significant increase in expression of the main osteoblastic differentiation genes: BMP2, alkaline phosphatase, osteocalcin, COL1A1, RUNX2. The monitoring within 96 hours demonstrated a progressive increase of cell adhesion and an intense cell proliferation at 48 h. The differentiation response and proliferation of stem cells after treatment with SW shows that this therapy is an effective method of regenerative medicine.


Keywords

shock waves;stem cell;differentiation;bone


References

Supporting Agencies



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