Heat shock induces the expression of pro-inflammatory cytokines in human Achilles tendon tenocytes

D Somma, S Formisano, D Rosa, S L Di, N Maffulli

Article ID: 6584
Vol 30, Issue 4S1, 2016
DOI: https://doi.org/10.54517/jbrha6584
Received: 8 January 2017; Accepted: 8 January 2017; Available online: 8 January 2017; Issue release: 8 January 2017

Abstract

The aim of our study is to investigate the behaviour of healthy and tendinopathic human tenocytes after a heat shock. After we harvested tendinopathic and healthy human tendon samples, we split tenocytes into 4 groups: 3 groups were submitted to heat shock, followed by different periods of post-heating (2, 4 and 20 h). The other group represents our negative control. The target genes were analysed using Real Time PCR. IL-1β and IL-6 expression were significantly increased in tendinopathic samples after heat shock. COL1 and COL3 expression were increased in non-stimulated tendinopathic tenocytes, but their levels significantly decreased after heat shock (p less than 0.01). COL3 levels increase in healthy samples after 20 h post-heating (p less than 0.01). COL1 and COL3 decreased after heat shock as a sign of the failure of repair mechanisms in tendinopathic tendons. Heat shock in in vitro models was insufficient to trigger pro-inflammatory cytokines in healthy human tenocytes.


Keywords

Stress-related changes;pro-inflammatory cytokines;heat shock;in vitro;human tenocytes


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