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CHARACTERIZATION OF HEPATITIS B VIRUS INTEGRATION LANDSCAPE IN PATIENTS WITH HEPATOCELLULAR CARCINOMA AND IN PLC/PRF/5 CELL LINES
Vol 32, Issue 4S1, 2018
Abstract
HBV DNA integration into the host hepatocyte genome is an early event in HBV infection and itis found in about 85% of HCC. Most of the data on HBV integration in HCC have been generatedby specific restriction enzymes and cloning methods that may favor preferential amplification andbias identification of unique integration sites. On the other hand, a limitation of the more recent nextgenerationsequencing (NGSs) approaches is the low coverage of HBV reads. Aims. To conduct a highthroughputviral integration detection analysis on tumor (T) and non-tumor (NT) liver tissues, and onPLC/PRF/5 cells, using NGS of enriched HBV integrants. To characterize the HBV DNA integrationevents and enumerate clones of expanded hepatocytes that bear identical integrations. To identify HBVjunctures at the whole-transcriptome level and define the transcribed viral-human fusions.
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Medical Genetics, University of Torino Medical School, Italy
Department of Biomedical, Surgical and Dental Sciences, University of Milan, Italy