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Effect of magnesium ions on ethanol-induced changes in the pool of saturated, mono- and polyunsaturated fatty acids in isolated rat hepatocytes
Vol 26, Issue 4, 2012
Abstract
The aim of the paper was the assessment of the effect of magnesium ions on ethanol-induced changes in the content of ester-bound fatty acids in isolated rat hepatocytes and their cell membranes. Hepatocytes were isolated by means of the enzymatic method of Selgen using collagenase. The number and viability of isolated rat hepatocytes, incubated for 5 hours in culture media (Hepatocyte Medium) with ethanol and MgCl2 solutions with concentration amounting respectively to: 150 mM/dm3 of ethanol and/or 2 mM/dm3 of MgCl2, 4 mM/dm3 of MgCl2, were determined. Biochemical tests of hepatocytes were performed, consisting in the determination of the total content of ester-bound fatty acids in whole hepatocytes and their cell membranes after incubation. Confirmed normalising action of magnesium ions with respect to the effects induced in hepatocytes and their membranes by the presence of ethanol should be attributed to the important role of magnesium which it performs during reactions taking place with participation of ATP.
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Copyright (c) 2012 E. Grochowska-Niedworok, B. Calyniuk, M. Muc-Wierzgon, E. Nowakowska-Zajdel
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Medical Genetics, University of Torino Medical School, Italy
Department of Biomedical, Surgical and Dental Sciences, University of Milan, Italy