Comparative evaluation of gastric ghrelin cells and levels of hormone in the serum of healthy women and men

Abstract

Due to difficulties in obtaining human material, most of the data concerning the site of occurrence and synthesis of ghrelin are based on animal studies. There are only few reports describing ghrelin-containing cells in the human digestive tract, based on the limited human material obtained during surgery or biopsy. The aim of this study was to compare and evaluate the distribution and morphology of ghrelin cells in the stomach and the levels of hormone in the serum of healthy men and women. The study included 18 subjects with normal gastric mucosa (12 men and 6 women). Immunohistochemistry was performed using rabbit anti-ghrelin (human) antiserum. Ghrelin level in serum was measured by ELISA. The total number of ghrelin positive cells was greater in the stomach of women than men. Ghrelin-immunoreactive cells were more elongated and larger in the stomach of women. The serum ghrelin level was higher in men than in women. Ghrelin concentration in serum correlates negatively with body mass index and weight in both genders, whereas the correlation between ghrelin level and age was positive in women and negative in men. The number of cells containing ghrelin in the stomach does not reflect the serum hormone levels. The differences in gastric ghrelin cells and ghrelin levels in serum between women and men, indicate that secretion of hormone can be under control sex hormones or other unknown factors.