BERBERIS VULGARIS FRUIT CRUDE EXTRACT AS A NOVEL ANTI-LEUKAEMIC AGENT

T A Saedi, S Ghafourian, M Jafarlou, M N Sabariah, P Ismail, R M T, F Othman

Article ID: 5802
Vol 29, Issue 2, 2015
DOI: https://doi.org/10.54517/jbrha5802
Received: 9 July 2015; Accepted: 9 July 2015; Available online: 9 July 2015; Issue release: 9 July 2015

Abstract

Tumor protein p53 encoded by the TP53 gene in humans is known as a cancer biomarker in patients diagnosed with cancer, and it plays an essential role in apoptosis, genomic stability, and inhibition of angiogenesis. Cancer therapies with common chemotherapy methods are effective, as known, but have some side effects. Berberis vulgaris is traditionally administrated as a cancer drug. The current research aims to evaluate p53 as a biomarker in WEHI-3 cell line and to demonstrate the Berberis vulgaris fruit crude extract (BVFCE) as a new anticancer drug. For this purpose, we evaluated the effect of BVFCE in different concentrations against WEHI-3cell line in vitro and determined the quantitative level of p53 gene in the treated WEHI-3 cells. The results demonstrated that even at only 1 mg/ml concentration of Berberis vulgaris crude extract, there was a low level of p53 biomarker expression on WEHI-3 cells in comparison with doxorubicin. Therefore, the current study suggests BVFCE as a reliable anti-leukaemic drug and candidate for anticancer therapy. However, further investigation need be carried out to confirm its efficiency in vivo.


Keywords

BVFCE;anti-leukaemia;WEHI-3;qRT-PCR;p53


References

Supporting Agencies



Copyright (c) 2015 T A Saedi, S Ghafourian, M Jafarlou, M N Sabariah, P Ismail, R M T Eusni, F Othman




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