Cytological mechanism of astragaloside IV in promoting repair of bone defects

Abstract

To explore the possible cytological mechanism underlying the role of Astragaloside IV in promotingthe repair of bone defects, osteoblasts were cultured in vitro and identified using inverted phase contrastmicroscopy, alkaline phosphatase (ALP) staining and alizarin red staining. Subsequently, cells were subculturedinto 24-well plates with different concentrations (0.1 μg/mL, 1 μg/mL, 10 μg/mL, 100 μg/mL,and 1000 μg/mL) of Astragaloside IV before the content of ALP in the culture medium was determined.Using a similar method, a proliferative assay of the cells was carried out to determine the proliferationability of cultured osteoblasts. The results showed that 1μg/mL, 10μg/mL and 100μg/mL AstragalosideIV solutions promoted proliferation of osteoblasts, while 10μg/mL and 100μg/mL promoted the secretionof ALP by osteoblasts. In conclusion, Astragaloside IV of concentrations in a certain range can promotethe proliferation and differentiation of osteoblasts, suggesting the presence of a cytological mechanismunderlying its role in promoting the repair of bone defects.