Effect of propranolol on proliferation and apoptosis of hemangioma endothelial cells in infants and young children

Abstract

The aim of this study was to investigate the effects of propranolol on the proliferation and apoptosis of hemangioma endothelial cells in infants and young children, and to explore the molecular mechanism of hemangioma treatment. Infant HemEC was cultured in vitro. HemEC cells were treated with different concentrations of propranolol (0umol/L, 25umol/L, 50umol/L, 75umol/L, 100umol/L, 125umol/L). After 24, 48 and 72 hours, the viability of the cells was examined by MTT {3-(4, 5-Dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide} method. The apoptosis rate of the cells was measured by flow cytometry using Annexin V. The propranolol concentration was 25umol/L. After 24 h and 48 h, HemEC could slightly proliferate (P<0.05). With the concentration of ≥100umol/L, the survival time of HemEC decreased when the action time was longer than 24 h. Within a certain range, the drug efficacy was positively correlated with drug concentration and action time (P<0.05). When propranolol concentration was ≥100umol/L, it could cause HemEC apoptosis. With the increase of drug concentration and the prolongation of intervention time, the apoptosis rate increased (P<0.05). In conclusion, the inhibition of hemangiomas by propranolol may be related to the inhibition of HemEC proliferation and its promotion of apoptosis.