Effect of zinc, copper and resveratrol supplementation on the urinary level of 15-hydroxyeicosatetraenoic acid at the early stage of breast cancer in rats

B. Bobrowska-Korczak, D. Skrajnowska, K. Seidel, W. Bielecki, B. Gadzinski

Article ID: 4515
Vol 33, Issue 1, 2019
DOI: https://doi.org/10.54517/jbrha4515
Received: 20 August 2018; Accepted: 20 August 2018; Available online: 11 March 2019; Issue release: 11 March 2019

Abstract

The aim of the present study was to assess the effect of zinc and copper, separately or in combination with resveratrol, on the concentration of 15-hydroxyeicosatetraenoic acid (15-HETE) in urine of rats with mammary cancer (adenocarcinoma) induced with 7,12- dimethyl-1,2-benz[a]anthracene (+ DMBA). The research focused on the kinetics of alterations in urinary 15-HETE at the early stage of carcinogenesis, as well as on the influence of dietary factors on the process. The content of 15-hydroxyeicosatetraenoic acid in the rats urine was determined by enzyme-linked immunosorbent assay (ELISA). The 15-HETE level was standardized by conversion to the creatinine level. Regardless of the diet (standard, Zn, Zn+resveratrol, Cu, Cu+resveratrol), DMBA-induced breast carcinogenesis was not inhibited. On the contrary, in the Zn+resveratrol supplemented group, tumorigenesis developed at a considerably faster rate. It was found that the supplementation of the rats diet with zinc only or zinc plus resveratrol resulted in a reduction of the 15-HETE level in urine of rats with mammry cancer (+ DMBA) as compared with control rats (- DMBA). A statistically lower concentration of 15-HETE was found in urine of rats supplemented with zinc or copper with resveratrol when compared with the animals that received only zinc or copper (p=0.02; p=0.0001). Summing up the obtained results, it can be concluded that the level of 15-HETE in urine of the examined animals depended on the applied supplementation.


Keywords

15-HETE;cancer;zinc;cooper;resveratrol


References

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