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miR-449a inhibits cell viability of human prostate cancer cells by suppressing Notch1 expression and promoting cell autophagy
Vol 35, Issue 6, 2021
Abstract
Prostate cancer is a commonly seen male cancer in the world. In the present study, we explored therole of Notch1 and how it is regulated in the progression of prostate cancers. Firstly, Notch1 was highlyexpressed in prostate cancer cell lines PC3 and Du145, compared with normal human prostate cellcontrol cell RWPE-1. Knockdown Notch1 by specific shRNA significantly suppressed cell proliferationof PC3 cells. Has-miR-449a is found to negatively regulate the expression of Notch1 in human prostatecancer cells. Moreover, MiR-449a mimics suppresses cell viability of PC3 cells, and miR-449a inhibitorpromotes cell proliferation of PC3 cells. Furthermore, transfection with miR-449a decreased the levelof Notch1 and increased the autophagy activity of human prostate cancer cells. In conclusion, miR-449a negatively regulates the expression of Notch1 and inhibits the cell proliferation of human prostatecancer cells partly due to increasing the cell autophagy of PC3 cells
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Copyright (c) 2021 Q. Niu, H Zhang, YJ Zhang, XW Song, L Dong
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Medical Genetics, University of Torino Medical School, Italy
Department of Biomedical, Surgical and Dental Sciences, University of Milan, Italy