Hsa_circ_0005075 promotes the proliferation, migration, and invasion of colorectal cancer cells via miR-431/DDX5 axis

Abstract

Circular RNAs (circRNAs) serve as important regulators in cancer biology. The goal of the presentresearch is to investigate the biological functions and mechanism of circ_0005075 in colorectalcancer (CRC). The expressions of circ_0005075, miR-431, and DDX5 mRNA were quantified in CRCtissues and cell lines by quantitative real-time polymerase chain reaction (qRT-PCR). Cell countingkit-8 (CCK-8) assay, Transwell assay, and flow cytometry were conducted for assessing CRC cellproliferation, migration, invasion, cell cycle distribution, and apoptosis. Bioinformatics analysis andluciferase reporter assay were used for identifying the binding site between circ_0005075 and miR-431.Western blotting was employed for the quantification of DDX5, E-cadherin, N-cadherin, and Vimentinprotein expressions. The upregulation of circ_0005075 expression and the down-regulation of miR-431 expression were observed in CRC tissues and cell lines. Knockdown of circ_0005075 triggered theinhibition of proliferation, migration, and invasion of CRC cells, induced cell cycle arrest in G0/G1phase, and promoted cell apoptosis. Circ_0005075 could function as a molecular sponge for miR-431. Inaddition, circ_0005075 could repress miR-431 expression to up-regulate DDX5 expression in CRC cells.Circ_0005075, regulating miR-431/DDX5 pathway, promotes CRC progression