Proliferative, anti-apoptotic and immune-enhancing effects of L-arginine in culture of skin fibroblasts

H. Kocic, I. Arsic, M. Stankovic, D. Tiodorovic, V. Ciric, G. Kocic

Article ID: 4369
Vol 31, Issue 3, 2017
DOI: https://doi.org/10.54517/jbrha4369
Received: 6 June 2022; Accepted: 6 June 2022; Available online: 6 June 2022; Issue release: 6 June 2022

Abstract

Semi-essential amino acid L-arginine may be of fundamental importance in various intracellular and intercellular pathways related to skin repair and wound healing. Our current study was aimed to explore the effect of L-arginine on skin fibroblast (L929) signaling pathways involved in cell proliferation (Akt-pAkt kinase, Erk/pErk1/2 kinase, JNK/pJNK kinase and pStat-1), apoptosis (Bcl2 and Bax) and immune defense (NF-κB and CD26). Significant upregulation of Erk (p>0.011), pErk (p>0.017) and JNK (p>0.002) was documented, while the rise was not significant for pJNK kinase. The Akt/pAkt signaling pathway did not change significantly for the above-mentioned time and dose, while pStat-1 was significantly down regulated (p>0.011). The exposure of skin fibroblasts to L-arginine increased anti-apoptotic Bcl2/Bax stoichiometry ratio (p>0.05), obtained by calculation of their individual quantities. L-arginine was able to elicit NF-κB signaling through the increase of p65 active subunit level (p>0.004), while CD26 surface antigen level was not significantly changed. In conclusion, the exposure of skin fibroblasts to L-arginine may help in maintaining and stimulating skin fibroblast proliferative, anti-apoptotic and immune defense function. Therefore, the proposed L-arginine dose may be used for tissue regeneration application, which would be of importance in regenerative medicine, skin rejuvenation approaches and wound healing.


Keywords

L-arginine;Akt kinase;Erk kinase;JNK kinase;pStat-1 kinase;Bcl2;Bax;NF-κB;CD26


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