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Penehyclidine hydrochloride postconditioning ameliorates cerebral ischemia-reperfusion injury: critical role of mitochondrial ATP sensitive potassium channel
Vol 30, Issue 1, 2016
Abstract
Cerebral ischemia/reperfusion (CI/R) leads to disability and death worldwide. However, limited approaches have been made in developing therapies to decrease the detrimental effects of CI/R. In the present study, we evaluated the effects of penehyclidine hydrochloride (PHC) postconditioning on CI/R injury and elucidated the underlying mechanisms. In CI/R rats, we showed that PHC postconditioning could effectively inhibit I/R-induced brain infarction and edema, and deficit of neurological function. In addition, PHC postconditioning significantly inhibited I/R-induced increase of proinflammatory cytokines and TUNEL-positive cell numbers. Moreover, the opening of mitochondrial permeability transition pore (mPTP) and oxidative stress resulting from I/R were suppressed notably by PHC postconditioning, as evidenced by increased absorbance at 540 nm in Ca2+-induced mitochondrial swelling test and increased SOD activity and decreased ROS and MDA level. 5-hydroxydecanoate (5-HD), a specific inhibitor of MitoKATP, was used to evaluate the role of MitoKATP in the neuroprotective effect of PHC postconditioning. The results showed that 5-HD could markedly suppress PHC postconditioning-caused inhibition of brain infarction and edema, deficit of neurological function, inflammation, apoptosis, opening of mPTP and oxidative stress induced by CI/R. The results demonstrated that PHC postconditioning could protect against I/R injury in brain through the inhibition of oxidative stress, mPTP opening, apoptosis and inflammation. Activation of MitoKATP was critical for PHC postconditioning-exhibited neuro-protective effects against I/R injury. These findings may provide a novel foundation for therapeutic strategies targeting cerebral protection against I/R damage.
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Copyright (c) 2016 Y. Shu, Z. Li, B. Han
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Medical Genetics, University of Torino Medical School, Italy

Department of Biomedical, Surgical and Dental Sciences, University of Milan, Italy