Acute effects of n-3 polyunsaturated fatty acids (PUFAs) reducing tumor necrosis factor-alpha (TNF-a) levels and not lowering malondialdehyde (MDA) levels after anaerobic exercise

Abstract

This study aimed to analyze the acute effect of n-3 PUFA on TNF-a and MDA levels after anaerobic exercise. This experimental study involved 16 healthy adult men randomly selected with a BMI range of 19.00-24.00, and age ranged from 20-30 years. Subjects were selected randomly and then divided into 2 groups: group K1 using placebo and group K2 using n-3 PUFAs at a dose of 540 mg EPA and 360 DHA. The intervention was carried out 24 hours after the research subjects completed the anaerobic exercise. The data in this study were TNF-a levels measured using a human ELISA kit, and MDA was measured using a spectrophotometer taken before and after the intervention. The test of different levels of TNF-a in K2 (p<0.05) p=0.027, K1 (p>0.05) p=0.327, so that n-3 PUFAs significantly reduced TNF-a levels and the test of different levels of MDA in the group K2 (p> 0.05) p = 0.511, K2 (p> 0.05) p = 0.541 so that n-3 PUFAs did not significantly reduce MDA levels. Thus, it can be concluded that the administration of n-3 PUFAs has been shown to reduce TNF-a levels without a decrease in Malondialdehyde (MDA) levels after anaerobic exercise.