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Effect of Panaxadiol on gene expression in rat cardiovascular endothelial cells based on RNA sequencing

Dongyang Guo, Xiufang Hong, Lingzhi Shen, Genxiang Mao, Zhouxin Yang

Article ID: 1914
Vol 3, Issue 1, 2022

DOI: https://doi.org/10.54517/ccr.v3i1.1914
VIEWS - 38 (Abstract)

Abstract

Objective: To systematically analyze the effect of Panaxadiol on gene expression of cardiac microvascular endothelial cells (cmecs) during lipopolysaccharide (LPS) infection. Methods by isolating primary cmecs from rats, the cells were divided into control group and experimental group. The control group was stimulated by LPS alone, and the experimental group was stimulated by LPS and Panaxadiol together. After that, the differentially expressed genes and related signal pathways were analyzed by transcriptome sequencing, and some differentially expressed genes were verified by quantitative PCR. Results Panaxadiol could significantly inhibit the expression of some genes, which were mainly concentrated in inflammatory signal related pathways, metabolic related pathways and epigenetic regulation. Conclusion: Panaxadiol may affect the expression of inflammation related genes through epigenetic regulation, and then regulate the inflammatory response of cmecs.

Keywords

lipopolysaccharide; cardiac microvascular endothelial cells; panaxadiol; transcriptome sequencing

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