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A novel method for glucose detection using dual-modal carbon dots for colorimetric and ratiometric fluorescence analysis
Vol 5, Issue 1, 2024
Issue release: 30 June 2024
VIEWS - 4217 (Abstract)
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Abstract
Iron-based nitrogen co-doped carbon dots (Fe, N-CDs) were synthesized from taro leaf biomass via a hydrothermal process using ammonium ferric sulfate dodecahydrate and urea. The synthesized Fe, N-CDs exhibited peroxidase-like activity and strong fluorescence at 450 nm. A dual-mode colorimetric/ratiometric fluorescence assay for hydrogen peroxide (H2O2) detection was developed using Fe, N-CDs and o-phenylenediamine (OPD) as probes. In the presence of H2O2, OPD was oxidized to 2,3-diaminophenazine (DAP), which is yellow and absorbs at 420 nm. Under 360 nm excitation, DAP emits fluorescence at 550 nm and quenches the fluorescence of Fe, N-CDs at 450 nm due to the internal fluorescence filtering effect. This enables the quantitative analysis of H2O2 using the absorbance at 420 nm (A420) and the fluorescence intensity ratio of DAP to Fe, N-CDs (I550/I450). Since glucose oxidase can convert glucose to H2O2, the assay was extended to glucose determination. At pH 5.4, 40 ℃, with 1.75 mmol/L OPD and a 25-minute reaction time, the method showed a linear relationship between the A420 and I550/I450 values and glucose concentration in the range of 1.0~100 μmol/L, with detection limits of 0.8 μmol/L (colorimetry) and 0.6 μmol/L (ratiometry). The method was validated for glucose detection in human serum.
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Prof. Sivanesan Subramanian
Anna University, India
Prof. Pascal Lorenz
University of Haute Alsace, France
Dortmund University of Technology, Germany.
Interests: Mass spectrometry, Molecular Structural Analysis, Methodology; Application; Biological, Environmental and Food samples.
