Determination of four sulfonamides residues in prawn by ultrasound-assisted matrix solid phase dispersive extraction combined with pre-column derivation high performance liquid chromatography-fluorescence
Vol 4, Issue 1, 2023
VIEWS - 50 (Abstract)
Abstract
Objective To establish a method for the simultaneous determination of four sulfonamides residues in prawn, including sulfadiazine, sulfathiazole, sulfamerazine and sulfamethazine, by ultrasound-assisted matrix solid phase dispersion extraction coupled with pre - column derivation high performance liquid chromatography(HPLC). Methods Through the optimization of extraction conditions, ethyl acetate was selected as the extraction agent and florisil as the solid dispersion agent, and the sulfonamides in prawn were extracted with the method ultrasound-assisted matrix solid phase dispersion. The sulfonamides were pre-column derived by fluorescamine and detected by HPLC-fluorescence method. Results All sulfonamides showed good linearity in the concentration range of 2-100μg/L, with the correlation coefficient>0.999. The limit of detection and limit of quantification was 0.5 and 2μg/kg, respectively. The spike recoveries of blank prawn samples were 84.4%-93.9% at two levels of 2 and 20μg/kg, with the relative standard deviation(n =3)less than 7.7%. Conclusion The method is simple, time-consuming and high precision, which meets the requirements of residue analysis.
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