Determination of natural vitamin A in fish liver by liquid chromatography
Vol 3, Issue 1, 2022
VIEWS - 69 (Abstract)
Abstract
In order to accurately determine the content of vitamin A (vitamin A1, vitamin A2) in fish liver, sample pretreatment methods (water bath saponification, room temperature saponification, direct extraction) and detection methods (normal phase chromatography, reversed phase chromatography) were screened and applied to the determination of vitamin A in the liver of nine economic fish species. The results showed that vitamin A1 and vitamin A2 were separated effectively by reversed phase chromatography and showed good linear relationship within their respective linear ranges(R2 > 0.99); the content of vitamin A extracted by water bath saponification was significantly higher than that by room temperature saponification and direct extraction (p <0. 05), and its average recoveries of vitamin A1 and vitamin A2 were 104. 52% and 90. 94%, respectively. Except for the freshwater snakehead and big mouth bass, the total content of vitamin A in the livers of other freshwater fishes and marine fishes was more than 200μg/100 g, and the total content of vitamin A in the liver of marine giant grouper was the highest, reaching 14 413.78μg/100 g. The water bath saponification method combined with reversed phase chromatography has good precision and is suitable for the determination of vitamin A in fish liver.
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