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Determination of 16 polycyclic aromatic hydrocarbons in shellfish by HPLC-VWD/FLD
Vol 3, Issue 1, 2022
Issue release: 31 December 2022
VIEWS - 3200 (Abstract)
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Abstract
A method has been developed to analyze 16 polycyclic aromatic hydrocarbons (PAHs) in shellfish samples. The procedure involves ultrasonic extraction, separation using florisil and MIP-PAHs columns, concentration through vacuum evaporation and nitrogen blowing, and detection via HPLC-VWD/FLD. The pre-treatment method was optimized, and at spiked levels of 0.5, 1.5, and 2 μg/kg, the average recoveries of the 16 PAHs ranged from 58.1% to 89.6%, 60.6% to 88.1%, and 72.3% to 98.0%, with relative standard deviations (RSDs) of 0.23% to 11%, 1.3% to 13%, and 1.5% to 14%, respectively, in Mytilus coruscus. The detection limit for the PAHs was 0.5 μg/kg. This method was also applied to analyze 16 PAHs in Ostrea gigas, Meretrix meretrix, and Sinonovacula constricta.
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Prof. Sivanesan Subramanian
Anna University, India
Prof. Pascal Lorenz
University of Haute Alsace, France
Dortmund University of Technology, Germany.
Interests: Mass spectrometry, Molecular Structural Analysis, Methodology; Application; Biological, Environmental and Food samples.
