Determination of 16 polycyclic aromatic hydrocarbons in shellfish by HPLC-VWD/FLD

Dandan Yang, Feng Han, Yongfu Shi, Xuanyun Huang, Dongmei Huang, Youqiong Cai

Article ID: 1994
Vol 3, Issue 1, 2022
DOI: https://doi.org/10.54517/aas.v3i1.1994
Received: 23 February 2022; Accepted: 17 March 2022; Available online: 30 March 2022; Issue release: 31 December 2022


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Abstract

A method has been developed to analyze 16 polycyclic aromatic hydrocarbons (PAHs) in shellfish samples. The procedure involves ultrasonic extraction, separation using florisil and MIP-PAHs columns, concentration through vacuum evaporation and nitrogen blowing, and detection via HPLC-VWD/FLD. The pre-treatment method was optimized, and at spiked levels of 0.5, 1.5, and 2 μg/kg, the average recoveries of the 16 PAHs ranged from 58.1% to 89.6%, 60.6% to 88.1%, and 72.3% to 98.0%, with relative standard deviations (RSDs) of 0.23% to 11%, 1.3% to 13%, and 1.5% to 14%, respectively, in Mytilus coruscus. The detection limit for the PAHs was 0.5 μg/kg. This method was also applied to analyze 16 PAHs in Ostrea gigas, Meretrix meretrix, and Sinonovacula constricta.

Keywords

polycyclic aromatic hydrocarbons (pahs); HPLC; florisil; MIP-pahs; shellfish


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