Comparison of high performance liquid chromatography and spectrophotometry in the determination of chitosan content in water-soluble fertilizers
Vol 4, Issue 2, 2023
VIEWS - 71 (Abstract)
Abstract
Different analytical methods for the determination of chitosan content in water-soluble fertilizers by HPLC and spectrophotometry were established, and the hydrolysis conditions of chitosan were systematically studied. The results showed that the hydrolysis effect of 1+1 hydrochloric acid solution at 100℃ for 24h was the best. Linear range of HPLC with 1-phenyl-3-methyl-5-pyrazolone (PMP) as derivatizing agent. The detection limit and spiked recovery are 1~200 mg/l, 0.07 mg/l and 95~101% respectively, and the corresponding indicators of spectrophotometry are respectively 0~100μg, 0.47μg and 94%. Through comparison, the two methods have good consistency in the determination results of water-soluble fertilizer samples, and can meet the analysis requirements of chitosan content in water-soluble fertilizer. Among them, HPLC has fewer interference factors and higher efficiency. In addition, methyl fluorene chloroformate (Fmoc-Cl) was also used as a derivant to derive the hydrolysate of chitosan, and then detected by high performance liquid chromatography. It was found that the measured results were basically consistent with the above two methods, but the high performance liquid chromatography and spectrophotometry established in this study were not suitable for the determination of chitosan content in water-soluble fertilizer containing nitrate.
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DOI: https://doi.org/10.54517/aas.v4i2.1991
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