Fluorescence Determination of Anthrax Biomarker Based on Copper-doped Carbon Nanodots
Vol 2, Issue 1, 2021
VIEWS - 36 (Abstract)
Abstract
In this study, water-soluble copper-doped carbon nanodots(Cu-CDs) with high fluores- cence quantum yield were prepared by one-step hydrothermal method using citric acid and ethylenedi- amine as precursors and copper sulfate as metal dopants. A new method for the fluorescence determi- nation of anthrax biomarker based on copper-doped carbon nanodots was established based on the strong chelation of 2, 6-pyridinedicarboxylic acid(DPA) with carbon nanodots. Under the optimum experimental conditions, there were good linear relationships for the fluorescence quenching rate of Cu-CDs with DPA in the concentration ranges of 5-100 nmol/L(r2 = 0.9941) and 150-400 nmol/L(r2 = 0.9976), and the detection limit of the proposed method was 2.3 nmol/L. With the advantages of low cost, high specificity, high sensitivity and simple operation, the method has a good application prospect in detection of anthrax biomarker.
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