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Fluorescence-based detection of anthrax biomarkers using copper-doped carbon nanodots
Vol 4, Issue 1, 2023
VIEWS - 2131 (Abstract)
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Abstract
In this study, water-soluble copper-doped carbon nanodots (Cu-CDs) with high fluorescence quantum yield were synthesized using a one-step hydrothermal process with citric acid and ethylenediamine as precursors and copper sulfate as the dopant. A novel fluorescence-based method for detecting anthrax biomarkers was developed, leveraging the strong chelation of 2,6-pyridinedicarboxylic acid (DPA) with the carbon nanodots. Under optimal conditions, the fluorescence quenching rate of Cu-CDs with DPA exhibited excellent linearity in the concentration ranges of 5–100 nmol/L (r² = 0.9941) and 150–400 nmol/L (r² = 0.9976), with a detection limit of 2.3 nmol/L. The method's low cost, high specificity, sensitivity, and simplicity suggest promising potential for anthrax biomarker detection.
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Prof. Sivanesan Subramanian
Anna University, India
Prof. Pascal Lorenz
University of Haute Alsace, France
Dortmund University of Technology, Germany.
Interests: Mass spectrometry, Molecular Structural Analysis, Methodology; Application; Biological, Environmental and Food samples.
