Enhancement in secondary metabolites and arbutin content via gamma irradiation elicitation in Bergenia ciliata (Haw.) Sternb. callus
Vol 1, Issue 1, 2023
VIEWS - 86 (Abstract)
Abstract
In recent times, plants are known to be the novel sources for obtaining pharmacologically active drugs which are produced by them in response to the stress which in turn facilitate mutualistic and antagonistic interactions of the plant with its changing environment. Keeping in view the medicinal importance and vulnerability status of Bergenia ciliata in vitro cultures combined with mutagenesis prove to be better option for improvement of this plant. The reddish green callus obtained on Gamborg’s half strength medium supplemented with TDZ (2 mg/L) and NAA (2 mg/L) were irradiated with different doses of radiations and evaluated for various parameters. The maximum carotenoid content was found in 0.5 Gy treated callus while as maximum carbohydrates were present in 1.0 Gy treated callus (7.36 mg/g) as compared to non-irradiated callus (6.30 mg/g). The higher doses of gamma radiations (4.0 to 5.0 Gy) showed enhanced carbohydrate levels as compared to non-irradiated callus. The highest protein content was found in 3.0 Gy treated callus (41.1 mg/g) followed by 2.5 Gy treated callus (39.9 mg/g). The methanolic extracts of non-irradiated and irradiated callus were subjected to DPPH radical scavenging activity with maximum activity in 1.0 Gy treated sample (29.44%) followed by 1.5 Gy (23.86%). In the present study, the antioxidant enzyme activities were also evaluated (glutathione reductase, superoxide dismutase and ascorbate peroxidase) with the highest activity in 2.0 Gy treated callus as compared to non-irradiated callus. The dried callus was examined for quantification of alkaloids and tannins. Both alkaloids and tannins were significantly higher in 1.5 Gy treated callus. Gamma irradiations showed overall increase in the phenol content with highest concentration in 0.5 Gy treated callus (0.35 mg/mL). With regard to flavonoids, lower doses of gamma irradiation showed inhibitory effect on the flavonoid synthesis in the present study. The highest flavonoid content was found in 4.5 Gy (0.26 mg/mL) as compared to control (0.08 mg/mL). The maximum arbutin was found in 3.0 Gy treated sample (366.27 µg/g) while as maximum bergenin was found in non-irradiated samples (2.47 µg/g) which was analysed via HPLC.
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1. Li R, Bruneau AH, Qu R. Morphological mutants of St. Augustine grass induced by gamma ray irradiation. Plant Breeding 2010; 129: 412–416. doi: 10.1111/j.1439-0523.2009.01735.x
2. Vardhan PV, Shukla LI. Gamma irradiation of medicinally important plants and the enhancement of secondary metabolite production. International Journal of Radiation Biology 2017; 93(9): 967–979. doi: 10.1080/09553002.2017.1344788
3. Khalifa AM, Abd ElShafy E, Abu Khudir R, Gaafar RM. Infuence of gamma radiation and phenylalanine on secondary metabolites in callus cultures of milk thistle (Silybum marianum L.). Journal of Genetic Engineering and Biotechnology 2022; 20(1): 166. doi: 10.1186/s43141-022-00424-2
4. Rafi S, Kamili AN, Ganai BA, et al. In vitro culture and biochemical attributes of Bergenia ciliata (Haw.) Sternb. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences 2016; 88: 609–619. doi: 10.1007/s40011-016-0797-9
5. Vivekanandan OS. History of chemical mutagenesis and biological aspects of metal compounds, plant extracts and food additives. The Scitech Journal 2014; 1: 11.
6. Shah TM, Mirza JI, Haq MA, Ata BM. Induced genetic variability in chickpea (Cicer arietinum L.) II. Comparative mutagenic effectiveness and efficiency of physical and chemical mutagens. Pakistan Journal of Botany 2008; 40(2): 605–613.
7. Xie CX, Xu A, Wu LJ, et al. Comparison of base substitution in response to nitrogen ion implantation and Co-60 gamma ray irradiation in Escherichia coli. Genetic and Molecular Biology 2014; 27(2): 284–290.
8. Tetuya S, Kazuo Y, Hideyoshi H, Hiroyuki K. Base excision repair enzyme and nuclease suppresses mutagenesis caused by 8-hydroxy-dGTP. DNA Repair 2008; 7(1): 88–94.
9. Al-Sudany GAAH, Majeed WZ, Al-Aubeidi HJARA. Detection of gamma radiation effect induced by Cobelt-60 on Escherichia coli cells. Journal of Al-Nahrain University 2010; 13(3): 129–133. doi: 10.22401/JNUS.13.3.21
10. Rahimi MM, Bahrani A. Influence of gamma irradiation on some physiological characteristics and grain protein in Wheat (Triticum aestivum L.). World Applied Sciences Journal 2011; 15(5): 654–659.
11. Kovacs E, Keresztes A. Effect of gamma and UV-B/C radiation on plant cell. Micron 2002; 33(2): 199–210. doi: 10.1016/S0968-4328(01)00012-9
12. Kim JH, Baek MH, Chung BY, et al. Alterations in the photosynthetic pigments and antioxidant machineries of red pepper (Capsicum annuum L.) seedlings from gamma-irradiated seeds. Journal of Plant Biology 2004; 47: 314–321. doi: 10.1007/BF03030546
13. Wi SG, Chung BY, Kim JH, et al. Ultrastructural changes of cell organelles in Arabidopsis stem after gamma irradiation. Journal of Plant Biology 2005; 48(2): 195–200. doi: 10.1007/BF03030408
14. Dhanavel D, Gnanamurthy S, Girija M. Effect of gamma rays on induced chromosomal variation in cowpea Vigna unguiculata (L.) Walp. International Journal of Current Science 2012; 245–250.
15. Chopra VL. Mutagenesis: Investigating the process and processing the outcome for crop improvement. Current Science 2005; 89(2): 353–359.
16. Lee YI, Lee IS, Lim YP. Variation in sweet pototo regenerates from gamma rays irradiated embriogenic callus. Journal of Plant Biotechnology 2003; 4: 163–170.
17. Tshilenge-Lukanda L, Kalonji-Mbuyi A, Nkongolo KK, Kizungu RV. Effect of gamma irradiation on Morpho-Agronomic characteristics of groundnut (Arachis hypogaea L.). American Journal of Plant Sciences 2013; 4: 2186–2192. doi: 10.4236/ajps.2013.411271
18. Le KC, Ho TT, Paek KY, Park SY. Low dose gamma radiation increases the biomass and ginsenoside content of callus and adventitious root cultures of wild ginseng (Panax ginseng Mayer). Industrial Crops and Products 2019; 130: 16–24. doi: 10.1016/j.indcrop.2018.12.056
19. Alphonse M, Thiagarajan K, Fulzele DP, et al. Effect of gamma radiation on gentiopicroside production in Gentiana kurroo Royle in vitro cultures. Industrial Crops and Products 2022; 176: 114392. doi: 10.1016/j.indcrop.2021.114392
20. Khan SJ, Khan H, Khan RD, et al. Development of sugarcane mutants through in vitro mutagenesis. Pakistan Journal of Biological Sciences 2000; 3(7): 1123–1125. doi: 10.3923/pjbs.2000.1123.1125
21. Devmalkar VS, Murumkar CV, Salunkhe SM, Chavan SJ. Studies on pigment chlorophyll isolation and estimation of different bryophytes for their biochemical properties. Journal of Natural Product and Plant Resources 2014; 4(2): 56–61.
22. Ganai BA, Masood A, Zargar MA. Experimental Biochemistry, 1st ed. Kumar Publications; 2005. pp. 136.
23. Bradford MM. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry 1976; 72: 248–254. doi: 10.1006/abio.1976.9999
24. Bates LS, Waldren RP, Teare ID. Rapid determination of free proline for water-stress studies. Plant and Soil 1973; 39: 205–207. doi: 10.1007/BF00018060
25. Parray JA, Hamid R, Kamili AN, et al. Biological efficacy and radical scavenging potential of shikonin in Arnebia benthamii (Wall ex. G Don) Johnston. Industrial Crops and Products 2015; 74: 434–439. doi: 10.1016/j.indcrop.2015.04.040
26. Foyer CH, Halliwell B. The presence of glutathione and glutathione reductase in chloroplasts: A proposed role in ascorbic acid metabolism. Planta 1976; 133(1): 21–25. doi: 10.1007/BF00386001
27. Dhindsa R, Plumb-Dhindsa P, Thorpe TA. Leaf senescence: Correlated with increased level of membrane permeability, lipid peroxidation and decreased level of superoxide dismutase and catalase. Journal of Experimental Botany 1981; 32: 93–101. doi: 10.1093/jxb/32.1.93
28. Nakano Y, Asada K. Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Plant and Cell Physiology 1981; 22(5): 867–880. doi: 10.1093/oxfordjournals.pcp.a076232
29. Harborne JB. Phytochemical Methods. A Guide to Modern Techniques of Plant Analysis, 2nd ed. Chapman and Hall; 1984. pp. 1–31.
30. Mallick CP, Singh MB. Plant Enzymology and Histo Enzymology. Kalyani publishers; 1980. p. 286.
31. Graham HD. Stabilization of the Prussian blue color in the determination of polyphenols. Journal of Agriculture and Food Chemistry 1992; 40(5): 801–805. doi: 10.1021/jf00017a018
32. Chang CC, Yang MH, Wen HM, Chern JC. Estimation of total flavonoid content in Propolis by two complementary colorimetric methods. Journal of Food Drug Analysis 2002; 10(3): 178–182. doi: 10.38212/2224-6614.2748
33. Boros B, Jakabova S, Madarasz T, et al. Validated HPLC method for simultaneous quantitation of bergenin, arbutin, and gallic acid in leaves of different Bergenia species. Chromatographia 2014; 77: 1129–1135. doi: 10.1007/s10337-014-2624-x
34. Mohajer S, Taha RM, Lay MM, et al. Stimulatory effects of gamma irradiation on phytochemical properties, mitotic behaviour, and nutritional composition of Sainfoin (Onobrychis viciifolia Scop.). Scientific World Journal 2014; 2014: 854093. doi: 10.1155/2014/854093
35. Neelam D, Tabasum T, Husain SA, et al. Radiation sensitivity of cajanus cajan to gamma radiations. Journal of Food Processing & Technology 2014; 5: 12. doi: 10.4172/2157-7110.1000394
36. El-Shafey NM, Hassaneen RA, Gabr MMA, El-Sheihy O. Pre-exposure to gamma rays alleviates the harmful effect of drought on the embryo-derived rice calli. Australian Journal of Crop Science 2009; 3(5): 268–277.
37. Falahati A, Kazemitabar SK, Bahrami AR, et al. The study of gamma irradiation effects on drought tolerance in rice (Oryza sativa L.). Indian Journal of Crop Science 2007; 2(1): 155–158.
38. Hussein S, Ling APK, Lau CY, et al. Morphological and biochemical responses of Eurycoma longifolia callus to gamma irradiation. Bioremediation, Biodiversity and Bioavailability 2012; 6: 70–74.
39. Cho Y, Song KB. Effect of gamma irradiation on the molecular properties of BSA and lactoglobulin. Journal of Molecular Biology 2000; 33: 133–137.
40. Sheikhi A, Hassanpour H, Jonoubi P, et al. The effect of gamma irradiation on in vitro total phenolic content and antioxidant activity of Ferula gummosa Bioss. Journal of Medicinal Plants 2016; 15(59): 123–131.
41. Esfandiari E, Shekari F, Shekari F, Esfandiari M. The effect of salt stress on antioxidant enzymes activity and lipid peroxidation on the wheat seedling. Notulae Botanicae Horti Agrobotanici Cluj-Napoca 2007; 35(1): 48–56. doi: 10.15835/nbha351251
42. El-Beltagi HS, Ahmed OK, El-Desouky W. Effect of low doses γ-irradiation on oxidative stress and secondary metabolites production of rosemary (Rosmarinus officinalis L.) callus culture. Radiation Physics and Chemistry 2011; 80(9): 968–976. doi: 10.1016/j.radphyschem.2011.05.002
43. Aly AAE. Biosynthesis of phenolic compounds and water soluble vitamins in Culantro (Eryngium foetidum L.) plantlets as affected by low doses of gamma irradiation. Analele Universităţii din Oradea: Fascicula Biologie 2010; 2017(2): 356–361.
44. Oufedjikh H, Mahrouz M, Amiot MJ, Lacroix M. Effect of gamma-irradiation on phenolics compounds and phenylalanine ammonia-lyase activity during storage in relation to peel injury from peel of Citrus clementina hort, Ex. tanaka. Journal of Agricultural and Food Chemistry 2000; 48(2): 559–565. doi: 10.1021/jf9902402
45. Variyar PS, Limaye A, Sharma A. Radiation-induced enhancement of antioxidant contents of soybean (Glycine max Merrill). Journal of Agricultural and Food Chemistry 2004; 52(11): 3385–3388. doi: 10.1021/jf030793j
46. Siddhuraju P, Makkar HPS, Becker K. The effect of ionizing radiation on antinutritional factors and the nutritional value of plant materials with reference to human and animal food. Food Chemistry 2002; 78(2): 187–205. doi: 10.1016/S0308-8146(01)00398-3
47. Patil A, Suryavanshi P, Fulzele D. In vitro regeneration of gamma irradiated callus of artemisia annua and evaluation of increase artemisinin content by HPLC analysis. Journal of Analytical & Pharmaceutical Research 2018; 7(5): 569‒573. doi: 10.15406/japlr.2018.07.00284
48. Abdelaleem MA, Elbassiony KRA. Evaluation of phytochemicals and antioxidant activity of gamma irradiated quinoa (Chenopodium quinoa). Brazilian Journal of Biology 2021; 81(3): 806–813. doi: 10.1590/1519-6984.232270
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