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Effects of two myricetin on renal fibrosis in diabetic nephropathy rats
Vol 4, Issue 1, 2023
VIEWS - 3891 (Abstract)
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Abstract
Objective: To investigate the protective effect of two - myricetin (DMY) on diabetic nephropathy (DN) and its effect on renal fibrosis. Methods: the DN model was established by intraperitoneal injection of high-dose (55 mg · kg-1) streptozotocin (STZ). The model was successfully established with blood glucose > 16.7 mmol · L-1. The rats were randomly divided into normal group, model group and low, medium and high dose DMY group (125, 250 and 500 mg · kg-1). DMY group was administered by gavage, and the model group was administered by normal saline for 12 weeks. The contents of 24h urinary protein (24h-Pro), blood urea nitrogen (BUN) and blood creatinine (SCR) were detected by biochemistry. He staining was used to observe the morphological changes of kidney in each group, PAS staining was used to observe the changes of basement membrane thickness of renal tissue in each group, and Masson staining was used to observe the degree of glomerulosclerosis and renal interstitial fibrosis in each group. TGF in renal tissue of rats in each group was detected by Western blot- β1. Expression level of Smad2 and Smad7. Results: compared with the normal group, the contents of 24h pro, bun and SCR in DN model group were significantly increased (P < 0.01). Compared with the model group, the contents of 24h pro, bun and SCR in each dose of DMY group decreased (P < 0.01). The pathological results showed that the renal tissue of the model group showed increased glomerular volume and glue more
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Prof. Wei-Yen Hsu
National Chung Cheng University, Taiwan
